Abstract
With the rapid advances in single-molecule and live-cell imaging studies to investigate biological problems, the role of chemical probes to monitor reactions in a live cell has considerably increased. However, selective labeling of a target protein or a specific residue is highly challenging due to the high complexity of the biological system. In particular, biological macromolecules (such as proteins, DNA, or RNA) share many functional groups that potentially cross-react with exogenous chemical probes. Thus, there are high demands for perfect biocompatible reactions utilizing biologically unavailable chemistry. Metal-catalyzed reactions have been extensively investigated as synthetic methodology studies, including initial attempts in applying the chemistry in aqueous solutions in vitro or even in biological conditions. Herein, the latest developments and progress in metal-catalyzed bio-orthogonal reactions for biomolecule labeling are summarized.
| Original language | English |
|---|---|
| Pages (from-to) | 1498-1507 |
| Number of pages | 10 |
| Journal | ChemBioChem |
| Volume | 20 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2019 Jun 14 |
| Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by intramural funding from KIST (2V06370-2E28030/CAP-16-02-KIST) and the Bio & Medical Technology Development Program of the National Research Foundation funded by the Ministry of Science, ICT & Future Planning (2017M3A9D8029942, 2018M3A9H4079286).
Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Keywords
- biocatalysis
- click chemistry
- conjugation
- cross-coupling
- organometallic catalysis
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Organic Chemistry