TY - JOUR
T1 - Recent progress in the engineering of C1-utilizing microbes
AU - Bae, Jiyun
AU - Jin, Sangrak
AU - Kang, Seulgi
AU - Cho, Byung Kwan
AU - Oh, Min Kyu
N1 - Funding Information:
This work was supported by the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) of the Republic of Korea ( NIBR202221102 ).
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/12
Y1 - 2022/12
N2 - The global climate crisis has led to the transition toward the sustainable production of chemicals and fuels with a low carbon footprint. Microbial utilization of one-carbon (C1) substrates, such as carbon dioxide, carbon monoxide, methane, formate, and methanol, may be a promising replacement for the current fossil fuel-based industry. However, natural C1-utilizing microbes are currently unsuitable for industrial applications because of their slow growth and low carbon conversion efficiency, which results in low productivity and yield. Here, we review the recent achievements in engineering C1-utilizing microbes with improved carbon assimilation efficiency and describe the development of synthetic microorganisms by introducing natural C1 assimilation pathways in non-C1-utilizing microbes. Finally, we outline the future directions for realizing the industrial potential of C1-utilizing microbes.
AB - The global climate crisis has led to the transition toward the sustainable production of chemicals and fuels with a low carbon footprint. Microbial utilization of one-carbon (C1) substrates, such as carbon dioxide, carbon monoxide, methane, formate, and methanol, may be a promising replacement for the current fossil fuel-based industry. However, natural C1-utilizing microbes are currently unsuitable for industrial applications because of their slow growth and low carbon conversion efficiency, which results in low productivity and yield. Here, we review the recent achievements in engineering C1-utilizing microbes with improved carbon assimilation efficiency and describe the development of synthetic microorganisms by introducing natural C1 assimilation pathways in non-C1-utilizing microbes. Finally, we outline the future directions for realizing the industrial potential of C1-utilizing microbes.
UR - http://www.scopus.com/inward/record.url?scp=85140988762&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2022.102836
DO - 10.1016/j.copbio.2022.102836
M3 - Review article
C2 - 36334444
AN - SCOPUS:85140988762
SN - 0958-1669
VL - 78
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
M1 - 102836
ER -
