Recent Research Trends in Stem Cells Using CRISPR/Cas-Based Genome Editing Methods

Da Eun Yoon; Hyunji Lee; Kyoungmi Kim

doi:10.15283/IJSC23030

Recent Research Trends in Stem Cells Using CRISPR/Cas-Based Genome Editing Methods

Da Eun Yoon
, Hyunji Lee^*
, Kyoungmi Kim^*

^*Corresponding author for this work

Department of Biomedical Sciences

Research output: Contribution to journal › Review article › peer-review

5 Citations (Scopus)

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR) system, a rapidly advancing genome editing technology, allows DNA alterations into the genome of organisms. Gene editing using the CRISPR system enables more precise and diverse editing, such as single nucleotide conversion, precise knock-in of target sequences or genes, chromosomal rearrangement, or gene disruption by simple cutting. Moreover, CRISPR systems comprising transcriptional activators/repressors can be used for epigenetic regulation without DNA damage. Stem cell DNA engineering based on gene editing tools has enormous potential to provide clues regarding the pathogenesis of diseases and to study the mechanisms and treatments of incurable diseases. Here, we review the latest trends in stem cell research using various CRISPR/Cas technologies and discuss their future prospects in treating various diseases.

Original language	English
Journal	International Journal of Stem Cells
Volume	17
Issue number	1
DOIs	https://doi.org/10.15283/IJSC23030
Publication status	Published - 2024

Bibliographical note

Publisher Copyright:
© 2024 by the Korean Society for Stem Cell Research

Keywords

Clustered regularly interspaced short palindromic repeats
Genome editing
Stem cell
Transcriptional regulator

ASJC Scopus subject areas

Developmental Biology
Cell Biology

Access to Document

10.15283/IJSC23030

Cite this

@article{5e7854217b9345f08249a15433d51ecb,

title = "Recent Research Trends in Stem Cells Using CRISPR/Cas-Based Genome Editing Methods",

abstract = "The clustered regularly interspaced short palindromic repeats (CRISPR) system, a rapidly advancing genome editing technology, allows DNA alterations into the genome of organisms. Gene editing using the CRISPR system enables more precise and diverse editing, such as single nucleotide conversion, precise knock-in of target sequences or genes, chromosomal rearrangement, or gene disruption by simple cutting. Moreover, CRISPR systems comprising transcriptional activators/repressors can be used for epigenetic regulation without DNA damage. Stem cell DNA engineering based on gene editing tools has enormous potential to provide clues regarding the pathogenesis of diseases and to study the mechanisms and treatments of incurable diseases. Here, we review the latest trends in stem cell research using various CRISPR/Cas technologies and discuss their future prospects in treating various diseases.",

keywords = "Clustered regularly interspaced short palindromic repeats, Genome editing, Stem cell, Transcriptional regulator",

author = "Yoon, \{Da Eun\} and Hyunji Lee and Kyoungmi Kim",

note = "Publisher Copyright: {\textcopyright} 2024 by the Korean Society for Stem Cell Research",

year = "2024",

doi = "10.15283/IJSC23030",

language = "English",

volume = "17",

journal = "International Journal of Stem Cells",

issn = "2005-3606",

publisher = "Sungkyunkwan University",

number = "1",

}

TY - JOUR

T1 - Recent Research Trends in Stem Cells Using CRISPR/Cas-Based Genome Editing Methods

AU - Yoon, Da Eun

AU - Lee, Hyunji

AU - Kim, Kyoungmi

PY - 2024

Y1 - 2024

N2 - The clustered regularly interspaced short palindromic repeats (CRISPR) system, a rapidly advancing genome editing technology, allows DNA alterations into the genome of organisms. Gene editing using the CRISPR system enables more precise and diverse editing, such as single nucleotide conversion, precise knock-in of target sequences or genes, chromosomal rearrangement, or gene disruption by simple cutting. Moreover, CRISPR systems comprising transcriptional activators/repressors can be used for epigenetic regulation without DNA damage. Stem cell DNA engineering based on gene editing tools has enormous potential to provide clues regarding the pathogenesis of diseases and to study the mechanisms and treatments of incurable diseases. Here, we review the latest trends in stem cell research using various CRISPR/Cas technologies and discuss their future prospects in treating various diseases.

AB - The clustered regularly interspaced short palindromic repeats (CRISPR) system, a rapidly advancing genome editing technology, allows DNA alterations into the genome of organisms. Gene editing using the CRISPR system enables more precise and diverse editing, such as single nucleotide conversion, precise knock-in of target sequences or genes, chromosomal rearrangement, or gene disruption by simple cutting. Moreover, CRISPR systems comprising transcriptional activators/repressors can be used for epigenetic regulation without DNA damage. Stem cell DNA engineering based on gene editing tools has enormous potential to provide clues regarding the pathogenesis of diseases and to study the mechanisms and treatments of incurable diseases. Here, we review the latest trends in stem cell research using various CRISPR/Cas technologies and discuss their future prospects in treating various diseases.

KW - Clustered regularly interspaced short palindromic repeats

KW - Genome editing

KW - Stem cell

KW - Transcriptional regulator

UR - https://www.scopus.com/pages/publications/85188237825

U2 - 10.15283/IJSC23030

DO - 10.15283/IJSC23030

M3 - Review article

AN - SCOPUS:85188237825

SN - 2005-3606

VL - 17

JO - International Journal of Stem Cells

JF - International Journal of Stem Cells

IS - 1

ER -

Recent Research Trends in Stem Cells Using CRISPR/Cas-Based Genome Editing Methods

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this