Reclassification of Serpens flexibilis Hespell 1977 as Pseudomonas flexibilis comb. nov., with Pseudomonas tuomuerensis Xin et al. 2009 as a later heterotypic synonym

Su Kyoung Shin, Chung Yeon Hwang, Yong Joon Cho, Hana Yi

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    9 Citations (Scopus)

    Abstract

    Serpens flexibilis was proposed in 1977 and approved in 1980 without the 16S rRNA gene sequence information. The sequence of S. flexibilis became available in 2010, after the publication of Pseudomonas tuomuerensis in 2009. Our preliminary phylogenetic analyses indicated that these two strains share high sequence similarity and therefore showed strong potential to be united into a single species. To clarify the taxonomic status of the two species, a polyphasic taxonomy study was conducted including whole genome sequencing. The value of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) between the genome sequences of S. flexibilis ATCC 29606T and P. tuomuerensis JCM 14085T were 98.1% and 89.0%, respectively. The phenotypic and chemotaxonomic properties including enzymatic activities, substrate utilization profiles, and fatty acids, supported that the two taxa have no pronounced difference and should thus constitute a single species. Therefore, we propose to transfer Serpens flexibilis Hespell 1977 to the genus Pseudomonas as Pseudomonas flexibilis comb. nov. (type strain = ATCC 29606T), with Pseudomonas tuomuerensis Xin et al. 2009 as a later heterotypic synonym of Pseudomonas flexibilis.

    Original languageEnglish
    Pages (from-to)563-566
    Number of pages4
    JournalSystematic and Applied Microbiology
    Volume38
    Issue number8
    DOIs
    Publication statusPublished - 2015

    Bibliographical note

    Funding Information:
    This work was supported by Basic Science Research Programs through the National Research Foundation of Korea (NRF-2013R1A1A3010041) and the Korea Polar Research Institute (PM14020).

    Keywords

    • Pseudomonas flexibilis
    • Pseudomonas tuomuerensis
    • Reclassification
    • Serpens flexibilis
    • Whole genome sequencing

    ASJC Scopus subject areas

    • Microbiology
    • Ecology, Evolution, Behavior and Systematics
    • Applied Microbiology and Biotechnology

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