Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes

Bobin Kang; Chae Young Kim; Jisu Hwang; Sojung Sun; Hyunwon Yang; Hyung Joo Suh; Hyeon Son Choi

doi:10.1007/s10068-019-00611-x

Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes

Bobin Kang, Chae Young Kim, Jisu Hwang, Sojung Sun, Hyunwon Yang, Hyung Joo Suh, Hyeon Son Choi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78%), while cytosolic NF-κB was increased (53%), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.

Original language	English
Pages (from-to)	1819-1828
Number of pages	10
Journal	Food Science and Biotechnology
Volume	28
Issue number	6
DOIs	https://doi.org/10.1007/s10068-019-00611-x
Publication status	Published - 2019 Dec 1

Bibliographical note

Funding Information:
This study was supported by a research grant from Seoul Women’s University (2019).

Publisher Copyright:
© 2019, The Korean Society of Food Science and Technology.

Keywords

Human monocyte (THP-1)
Inflammatory signaling
Monocyte-to-macrophage differentiation
Red ginseng extract (RGE)

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology

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10.1007/s10068-019-00611-x

Cite this

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title = "Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes",

abstract = "This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78%), while cytosolic NF-κB was increased (53%), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.",

keywords = "Human monocyte (THP-1), Inflammatory signaling, Monocyte-to-macrophage differentiation, Red ginseng extract (RGE)",

author = "Bobin Kang and Kim, {Chae Young} and Jisu Hwang and Sojung Sun and Hyunwon Yang and Suh, {Hyung Joo} and Choi, {Hyeon Son}",

note = "Funding Information: This study was supported by a research grant from Seoul Women{\textquoteright}s University (2019). Publisher Copyright: {\textcopyright} 2019, The Korean Society of Food Science and Technology.",

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AU - Kang, Bobin

AU - Kim, Chae Young

AU - Hwang, Jisu

AU - Sun, Sojung

AU - Yang, Hyunwon

AU - Suh, Hyung Joo

AU - Choi, Hyeon Son

PY - 2019/12/1

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AB - This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78%), while cytosolic NF-κB was increased (53%), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.

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Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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