TY - JOUR
T1 - Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes
AU - Kang, Bobin
AU - Kim, Chae Young
AU - Hwang, Jisu
AU - Sun, Sojung
AU - Yang, Hyunwon
AU - Suh, Hyung Joo
AU - Choi, Hyeon Son
N1 - Funding Information:
This study was supported by a research grant from Seoul Women’s University (2019).
Publisher Copyright:
© 2019, The Korean Society of Food Science and Technology.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78%), while cytosolic NF-κB was increased (53%), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.
AB - This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78%), while cytosolic NF-κB was increased (53%), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.
KW - Human monocyte (THP-1)
KW - Inflammatory signaling
KW - Monocyte-to-macrophage differentiation
KW - Red ginseng extract (RGE)
UR - http://www.scopus.com/inward/record.url?scp=85071421746&partnerID=8YFLogxK
U2 - 10.1007/s10068-019-00611-x
DO - 10.1007/s10068-019-00611-x
M3 - Article
AN - SCOPUS:85071421746
SN - 1226-7708
VL - 28
SP - 1819
EP - 1828
JO - Food Science and Biotechnology
JF - Food Science and Biotechnology
IS - 6
ER -