TY - JOUR
T1 - Reduction in acute ecotoxicity of paper mill effluent by sequential application of xylanase and laccase
AU - Dhiman, Saurabh Sudha
AU - Garg, Gaurav
AU - Sharma, Jitender
AU - Kalia, Vipin C.
AU - Kang, Yun Chan
AU - Lee, Jung Kul
PY - 2014/7/24
Y1 - 2014/7/24
N2 - In order to reduce the ecotoxicity of paper mill, four different enzymatic pretreatment strategies were investigated in comparison to conventional chemical based processes. In strategy I, xylanase-aided pretreatment of pulp was carried out, and in strategy II, xylanase and laccase-mediator systems were used sequentially. Moreover, to compare the efficiency of Bacillus stearothermophilus xylanase and Ceriporiopsis subvermispora laccase in the reduction of ecotoxicity and pollution, parallel strategies (III and IV) were implemented using commercial enzymes. Conventional CDEOPD 1D2 (CD, Cl2 with ClO2; EOP, H2O2 extraction; D1 and D 2, ClO2) and X/XLCDEOPD 1D2 (X, xylanase; L, laccase) sequences were employed with nonenzymatic and enzymatic strategies, respectively. Acute toxicity was determined by the extent of inhibition of bioluminescence of Vibrio fischeri with different dilutions of the effluent. Two-fold increase was observed in EC50 values for strategy I compared to the control process. On the other hand, sequential application of commercial enzymes resulted in higher acute toxicity compared to lab enzymes. In comparison to the control process, strategy II was the most efficient and successfully reduced 60.1 and 25.8% of biological oxygen demand (BOD) and color of effluents, respectively. We report for the first time the comparative analysis of the ecotoxicity of industrial effluents.
AB - In order to reduce the ecotoxicity of paper mill, four different enzymatic pretreatment strategies were investigated in comparison to conventional chemical based processes. In strategy I, xylanase-aided pretreatment of pulp was carried out, and in strategy II, xylanase and laccase-mediator systems were used sequentially. Moreover, to compare the efficiency of Bacillus stearothermophilus xylanase and Ceriporiopsis subvermispora laccase in the reduction of ecotoxicity and pollution, parallel strategies (III and IV) were implemented using commercial enzymes. Conventional CDEOPD 1D2 (CD, Cl2 with ClO2; EOP, H2O2 extraction; D1 and D 2, ClO2) and X/XLCDEOPD 1D2 (X, xylanase; L, laccase) sequences were employed with nonenzymatic and enzymatic strategies, respectively. Acute toxicity was determined by the extent of inhibition of bioluminescence of Vibrio fischeri with different dilutions of the effluent. Two-fold increase was observed in EC50 values for strategy I compared to the control process. On the other hand, sequential application of commercial enzymes resulted in higher acute toxicity compared to lab enzymes. In comparison to the control process, strategy II was the most efficient and successfully reduced 60.1 and 25.8% of biological oxygen demand (BOD) and color of effluents, respectively. We report for the first time the comparative analysis of the ecotoxicity of industrial effluents.
UR - http://www.scopus.com/inward/record.url?scp=84904818214&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0102581
DO - 10.1371/journal.pone.0102581
M3 - Article
C2 - 25058160
AN - SCOPUS:84904818214
SN - 1932-6203
VL - 9
JO - PloS one
JF - PloS one
IS - 7
M1 - e102581
ER -