Reflection phase microscopy using spatio-temporal coherence of light

Youngwoon Choi, Poorya Hosseini, Jeon Woong Kang, Sungsam Kang, Taeseok Daniel Yang, Min Gyu Hyeon, Beop Min Kim, Peter T.C. So, Zahid Yaqoob

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase microscope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and three-dimensional resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. A reflection phase microscope can potentially elucidate biomechanical mechanisms of pathological and physiological processes.

Original languageEnglish
Pages (from-to)1468-1473
Number of pages6
Issue number11
Publication statusPublished - 2018 Nov 20

Bibliographical note

Publisher Copyright:
© 2018 Optical Society of America.

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics


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