Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase microscope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and three-dimensional resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. A reflection phase microscope can potentially elucidate biomechanical mechanisms of pathological and physiological processes.
Bibliographical noteFunding Information:
Korea Health Industry Development Institute (KHIDI) Korea Health Technology R&D Project (HI14C3477); National Research Foundation of Korea (NRF) (2017R1C1B2010262); National Institutes of Health (NIH) (1R01HL121386-01A1, 4R44EB012415, 5R01NS051320, 9P41EB015871-26A1); National Science Foundation (NSF) (CBET-0939511); Hamamatsu Corporation; Singapore-MIT Alliance for Research and Technology Centre (SMART); BioSystems and Micromechanics (BioSyM); Korea University (KU) Future Research Grant.
© 2018 Optical Society of America.
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics