TY - JOUR
T1 - Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum
AU - Park, Yong Keun
AU - Diez-Silva, Monica
AU - Popescu, Gabriel
AU - Lykotrafitis, George
AU - Choi, Wonshik
AU - Feld, Michael S.
AU - Suresh, Subra
PY - 2008/9/16
Y1 - 2008/9/16
N2 - Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.
AB - Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.
KW - Erythrocyte
KW - Febrile temperature
KW - Malaria
KW - Mechanical properties
KW - Optical techniques
UR - http://www.scopus.com/inward/record.url?scp=52949115297&partnerID=8YFLogxK
U2 - 10.1073/pnas.0806100105
DO - 10.1073/pnas.0806100105
M3 - Article
C2 - 18772382
AN - SCOPUS:52949115297
SN - 0027-8424
VL - 105
SP - 13730
EP - 13735
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 37
ER -
