Regulation of CD40 reconstitution into a liposome using different ratios of solubilized LDAO to lipids

Kyung Eun Lee; Ho Min Kim; Jie Oh Lee; Hyesung Jeon; Sung Sik Han

doi:10.1016/j.colsurfb.2007.09.021

Regulation of CD40 reconstitution into a liposome using different ratios of solubilized LDAO to lipids

Kyung Eun Lee, Ho Min Kim, Jie Oh Lee, Hyesung Jeon, Sung Sik Han

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The integral membrane protein CD40 was found on the surface of B lymphocytes that interact with CD40L on T cells during the immune response. The hydrophobic transmembrane domains of membrane proteins can be stabilized in detergent or in lipid bilayers such as liposomes. Membrane proteins can be incorporated into the liposome in a similar fashion to the way they are handled in vivo. In this study, a large amount of full-sequence CD40 was produced using a bacterial system that contained a Mistic construct. The CD40 was then reconstituted into liposomes by detergent-mediated reconstitution. All stages in the process of liposome disruption with various detergent ratios were easily observed by monitoring the optical density. The structure of the liposome and the reconstitution of CD40 were confirmed by cryo-TEM. The results of the present study show that the detergent ratio had an effect on the structure of the liposome and the amount of CD40 that was reconstituted into the liposome.

Original language	English
Pages (from-to)	51-57
Number of pages	7
Journal	Colloids and Surfaces B: Biointerfaces
Volume	62
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfb.2007.09.021
Publication status	Published - 2008 Mar 15

Bibliographical note

Funding Information:
This work is supported by the Korea Basic Sciences Institute (RKB-2005-000-00007-0-2006) and Korea Research Institute of Standards and Science (M10326110002-04N0611-00210). H. Jeon is supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korean government (MOST) (no. M1042001000-04N2001-00110), and by the Molecular Imaging Program at the Korea Institute of Science and Technology.

Keywords

CD40
Cryo-TEM
LDAO solubilization
Liposome reconstitution
Membrane protein

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2007.09.021

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title = "Regulation of CD40 reconstitution into a liposome using different ratios of solubilized LDAO to lipids",

abstract = "The integral membrane protein CD40 was found on the surface of B lymphocytes that interact with CD40L on T cells during the immune response. The hydrophobic transmembrane domains of membrane proteins can be stabilized in detergent or in lipid bilayers such as liposomes. Membrane proteins can be incorporated into the liposome in a similar fashion to the way they are handled in vivo. In this study, a large amount of full-sequence CD40 was produced using a bacterial system that contained a Mistic construct. The CD40 was then reconstituted into liposomes by detergent-mediated reconstitution. All stages in the process of liposome disruption with various detergent ratios were easily observed by monitoring the optical density. The structure of the liposome and the reconstitution of CD40 were confirmed by cryo-TEM. The results of the present study show that the detergent ratio had an effect on the structure of the liposome and the amount of CD40 that was reconstituted into the liposome.",

keywords = "CD40, Cryo-TEM, LDAO solubilization, Liposome reconstitution, Membrane protein",

author = "Lee, {Kyung Eun} and Kim, {Ho Min} and Lee, {Jie Oh} and Hyesung Jeon and Han, {Sung Sik}",

note = "Funding Information: This work is supported by the Korea Basic Sciences Institute (RKB-2005-000-00007-0-2006) and Korea Research Institute of Standards and Science (M10326110002-04N0611-00210). H. Jeon is supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korean government (MOST) (no. M1042001000-04N2001-00110), and by the Molecular Imaging Program at the Korea Institute of Science and Technology.",

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AU - Lee, Kyung Eun

AU - Kim, Ho Min

AU - Lee, Jie Oh

AU - Jeon, Hyesung

AU - Han, Sung Sik

N1 - Funding Information: This work is supported by the Korea Basic Sciences Institute (RKB-2005-000-00007-0-2006) and Korea Research Institute of Standards and Science (M10326110002-04N0611-00210). H. Jeon is supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korean government (MOST) (no. M1042001000-04N2001-00110), and by the Molecular Imaging Program at the Korea Institute of Science and Technology.

PY - 2008/3/15

Y1 - 2008/3/15

N2 - The integral membrane protein CD40 was found on the surface of B lymphocytes that interact with CD40L on T cells during the immune response. The hydrophobic transmembrane domains of membrane proteins can be stabilized in detergent or in lipid bilayers such as liposomes. Membrane proteins can be incorporated into the liposome in a similar fashion to the way they are handled in vivo. In this study, a large amount of full-sequence CD40 was produced using a bacterial system that contained a Mistic construct. The CD40 was then reconstituted into liposomes by detergent-mediated reconstitution. All stages in the process of liposome disruption with various detergent ratios were easily observed by monitoring the optical density. The structure of the liposome and the reconstitution of CD40 were confirmed by cryo-TEM. The results of the present study show that the detergent ratio had an effect on the structure of the liposome and the amount of CD40 that was reconstituted into the liposome.

AB - The integral membrane protein CD40 was found on the surface of B lymphocytes that interact with CD40L on T cells during the immune response. The hydrophobic transmembrane domains of membrane proteins can be stabilized in detergent or in lipid bilayers such as liposomes. Membrane proteins can be incorporated into the liposome in a similar fashion to the way they are handled in vivo. In this study, a large amount of full-sequence CD40 was produced using a bacterial system that contained a Mistic construct. The CD40 was then reconstituted into liposomes by detergent-mediated reconstitution. All stages in the process of liposome disruption with various detergent ratios were easily observed by monitoring the optical density. The structure of the liposome and the reconstitution of CD40 were confirmed by cryo-TEM. The results of the present study show that the detergent ratio had an effect on the structure of the liposome and the amount of CD40 that was reconstituted into the liposome.

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KW - LDAO solubilization

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KW - Membrane protein

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Regulation of CD40 reconstitution into a liposome using different ratios of solubilized LDAO to lipids

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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