TY - JOUR
T1 - Released exopolysaccharide (r-EPS) produced from probiotic bacteria reduce biofilm formation of enterohemorrhagic Escherichia coli O157:H7
AU - Kim, Younghoon
AU - oh, Sejong
AU - Kim, Sae Hun
N1 - Funding Information:
This work was supported by a Grant (20050401-034-698-151-00-00) from BioGreen 21 Program, Rural Development Administration, Republic of Korea. Y. Kim was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-F00025).
PY - 2009/2/6
Y1 - 2009/2/6
N2 - Here, we characterized released-exopolysaccharides (r-EPS) from Lactobacillus acidophilus A4 with the goal of identifying natural compounds that represses biofilm formation. In plastic 96-well microplates that contained 1.0 mg/ml of r-EPS, enterohemorrhagic Escherichia coli (EHEC) biofilms were dramatically decreased by 87% and 94% on polystyrene and polyvinyl chloride (PVC) surfaces, respectively. In the presence of r-EPS, neither their growth rate nor their autoinducer-2-like activity was affected on the EHEC O157:H7. Importantly, consistent reduction in biofilm formation was also observed when r-EPS was applied to the continuous-flow chamber models. In addition, we found that adding r-EPS significantly repressed biofilm formation by affecting genes related to curli production (crl, csgA, and csgB) and chemotaxis (cheY) in transcriptome analysis. Furthermore, these r-EPS could prevent biofilm formation by a wide range of Gram-negative and -positive pathogens. This property may lead to the development of novel food-grade adjuncts for microbial biofilm control.
AB - Here, we characterized released-exopolysaccharides (r-EPS) from Lactobacillus acidophilus A4 with the goal of identifying natural compounds that represses biofilm formation. In plastic 96-well microplates that contained 1.0 mg/ml of r-EPS, enterohemorrhagic Escherichia coli (EHEC) biofilms were dramatically decreased by 87% and 94% on polystyrene and polyvinyl chloride (PVC) surfaces, respectively. In the presence of r-EPS, neither their growth rate nor their autoinducer-2-like activity was affected on the EHEC O157:H7. Importantly, consistent reduction in biofilm formation was also observed when r-EPS was applied to the continuous-flow chamber models. In addition, we found that adding r-EPS significantly repressed biofilm formation by affecting genes related to curli production (crl, csgA, and csgB) and chemotaxis (cheY) in transcriptome analysis. Furthermore, these r-EPS could prevent biofilm formation by a wide range of Gram-negative and -positive pathogens. This property may lead to the development of novel food-grade adjuncts for microbial biofilm control.
KW - Biofilm formation
KW - EHEC O157:H7
KW - Exopolysaccharide
KW - Lactobacillus acidophilus
UR - http://www.scopus.com/inward/record.url?scp=58249139318&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.12.053
DO - 10.1016/j.bbrc.2008.12.053
M3 - Article
C2 - 19103165
AN - SCOPUS:58249139318
SN - 0006-291X
VL - 379
SP - 324
EP - 329
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -