Remote ischemic preconditioning ameliorates indirect acute lung injury by modulating phosphorylation of IκBα in mice

Yun Hee Kim, Young Sung Kim, Byung Hwa Kim, Kuen Su Lee, Hyung Sun Park, Choon Hak Lim

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)


    Objective: Acute lung injury is responsible for mortality in seriously ill patients. Previous studies have shown that systemic inflammation is attenuated by remote ischemic preconditioning (RIPC) via reducing nuclear factor-kappa B (NF-κB). Therefore, we investigated whether lipopolysaccharide (LPS)-induced indirect acute lung injury (ALI) can be protected by RIPC. Methods: RIPC was accomplished by 10 minutes of occlusion using a tourniquet on the right hind limb of mice, followed by 10 minutes of reperfusion. This process was repeated three times. Intraperitoneal LPS (20 mg/kg) was administered to induce indirect ALI. Inflammatory cytokines in bronchoalveolar lavage fluid were analyzed using an enzyme-linked immunosorbent assay. Pulmonary tissue was excised for histological examination, and for examining NF-κB activity and phosphorylation of inhibitor of κBα (IκBα). Results: NF-κB activation and LPS-induced histopathological changes in the lungs were significantly alleviated in the RIPC group. RIPC reduced phosphorylation of IκBα in lung tissue of ALI mice. Conclusions: RIPC attenuates endotoxin-induced indirect ALI. This attenuation might occur through modification of NF-κB mediation of cytokines by modulating phosphorylation of IκBα.

    Original languageEnglish
    Pages (from-to)936-950
    Number of pages15
    JournalJournal of International Medical Research
    Issue number2
    Publication statusPublished - 2019 Feb 1

    Bibliographical note

    Funding Information:
    This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (No. NRF-2015R1A2A2A01006734).

    Publisher Copyright:
    © The Author(s) 2019.


    • Acute lung injury
    • cytokine
    • inflammation
    • ischemic preconditioning
    • mice
    • survival rate

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology
    • Biochemistry, medical


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