Remote Switching of Elastic Movement of Decorated Ligand Nanostructures Controls the Adhesion-Regulated Polarization of Host Macrophages

Ramar Thangam; Myeong Soo Kim; Gunhyu Bae; Yuri Kim; Nayeon Kang; Sungkyu Lee; Hee Joon Jung; Jinhyeok Jang; Hyojun Choi; Na Li; Minjin Kim; Sangwoo Park; Seong Yeol Kim; Thomas Myeongseok Koo; Hong En Fu; Yoo Sang Jeon; Andreja Ambriović-Ristov; Jae-Jun Song; Soo Young Kim; Steve Park; Qiang Wei; Changhyun Ko; Ki Bum Lee; Ramasamy Paulmurugan; Young Keun Kim; Heemin Kang

doi:10.1002/adfm.202008698

Remote Switching of Elastic Movement of Decorated Ligand Nanostructures Controls the Adhesion-Regulated Polarization of Host Macrophages

Ramar Thangam, Myeong Soo Kim, Gunhyu Bae, Yuri Kim, Nayeon Kang, Sungkyu Lee, Hee Joon Jung, Jinhyeok Jang, Hyojun Choi, Na Li, Minjin Kim, Sangwoo Park, Seong Yeol Kim, Thomas Myeongseok Koo, Hong En Fu, Yoo Sang Jeon, Andreja Ambriović-Ristov, Jae-Jun Song, Soo Young Kim, Steve ParkQiang Wei, Changhyun Ko, Ki Bum Lee, Ramasamy Paulmurugan, Young Keun Kim, Heemin Kang

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Design of materials with remote switchability of the movement of decorated nanostructures presenting cell-adhesive Arg-Gly-Asp ligand can decipher dynamic cell-material interactions in decorated ligand nanostructures. In this study, the decoration of ligand-bearing gold nanoparticles (ligand-AuNPs) on the magnetic nanoparticle (MNP) with varying ligand-AuNP densities is demonstrated, which are flexibly coupled to substrate in various MNP densities to maintain constant macroscopic ligand density. Magnetic switching of upward (“Upper Mag”) or downward (“Lower Mag”) movement of varying ligand-AuNPs is shown via stretching and compression of the elastic linker, respectively. High ligand-AuNP densities promote macrophage adhesion-regulated M2 polarization that inhibits M1 polarization. Remote switching of downward movement (“Lower Mag”) of ligand-AuNPs facilitates macrophage adhesion-regulated M2 polarization, which is conversely suppressed by their upward movement (“Upper Mag”), both in vitro and in vivo. These findings are consistent with human primary macrophages. These results provide fundamental understanding into designing materials with decorated nanostructures in both high ligand-AuNP density and downward movement of the ligand-AuNPs toward the substrate to stimulate adhesion-regulated M2 polarization of macrophages while suppressing pro-inflammatory M1 polarization, thereby facilitating tissue-healing responses.

Original language	English
Article number	2008698
Journal	Advanced Functional Materials
Volume	31
Issue number	21
DOIs	https://doi.org/10.1002/adfm.202008698
Publication status	Published - 2021 May 21

Bibliographical note

Funding Information:
R.T. and M.S.K. contributed equally to this work. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT) (No. 2020R1C1C1011038 and 2019R1A2C3006587). This work was also supported by a Korea University Grant. HAADF‐STEM imaging was conducted with the support of the Korea Basic Science Institute. This work made use of the EPIC facility of Northwestern University's NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS‐1542205), the MRSEC IRG2 program (NSF DMR‐1720139) at the Materials Research Center, the International Institute for Nanotechnology (IIN), the Keck Foundation, and the State of Illinois, through the IIN. The surgery was performed after obtaining approval from the Institutional Animal Care and Use Committee at Korea University for “KOREA‐2020‐0160” under the investigator of Prof. Jae‐Jun Song.

Funding Information:
R.T. and M.S.K. contributed equally to this work. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT) (No. 2020R1C1C1011038 and 2019R1A2C3006587). This work was also supported by a Korea University Grant. HAADF-STEM imaging was conducted with the support of the Korea Basic Science Institute. This work made use of the EPIC facility of Northwestern University's NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the MRSEC IRG2 program (NSF DMR-1720139) at the Materials Research Center, the International Institute for Nanotechnology (IIN), the Keck Foundation, and the State of Illinois, through the IIN. The surgery was performed after obtaining approval from the Institutional Animal Care and Use Committee at Korea University for ?KOREA-2020-0160? under the investigator of Prof. Jae-Jun Song.

Publisher Copyright:
© 2021 Wiley-VCH GmbH

Keywords

elastic ligand movement
macrophage adhesion
macrophage polarization
magnetic switching
nanoparticle decoration

ASJC Scopus subject areas

Chemistry(all)
Materials Science(all)
Condensed Matter Physics

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10.1002/adfm.202008698

Cite this

Thangam, R., Kim, M. S., Bae, G., Kim, Y., Kang, N., Lee, S., Jung, H. J., Jang, J., Choi, H., Li, N., Kim, M., Park, S., Kim, S. Y., Koo, T. M., Fu, H. E., Jeon, Y. S., Ambriović-Ristov, A., Song, J-J., Kim, S. Y., ... Kang, H. (2021). Remote Switching of Elastic Movement of Decorated Ligand Nanostructures Controls the Adhesion-Regulated Polarization of Host Macrophages. Advanced Functional Materials, 31(21), Article 2008698. https://doi.org/10.1002/adfm.202008698

Thangam, R, Kim, MS, Bae, G, Kim, Y, Kang, N, Lee, S, Jung, HJ, Jang, J, Choi, H, Li, N, Kim, M, Park, S, Kim, SY, Koo, TM, Fu, HE, Jeon, YS, Ambriović-Ristov, A, Song, J-J, Kim, SY, Park, S, Wei, Q, Ko, C, Lee, KB, Paulmurugan, R, Kim, YK & Kang, H 2021, 'Remote Switching of Elastic Movement of Decorated Ligand Nanostructures Controls the Adhesion-Regulated Polarization of Host Macrophages', Advanced Functional Materials, vol. 31, no. 21, 2008698. https://doi.org/10.1002/adfm.202008698

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title = "Remote Switching of Elastic Movement of Decorated Ligand Nanostructures Controls the Adhesion-Regulated Polarization of Host Macrophages",

abstract = "Design of materials with remote switchability of the movement of decorated nanostructures presenting cell-adhesive Arg-Gly-Asp ligand can decipher dynamic cell-material interactions in decorated ligand nanostructures. In this study, the decoration of ligand-bearing gold nanoparticles (ligand-AuNPs) on the magnetic nanoparticle (MNP) with varying ligand-AuNP densities is demonstrated, which are flexibly coupled to substrate in various MNP densities to maintain constant macroscopic ligand density. Magnetic switching of upward (“Upper Mag”) or downward (“Lower Mag”) movement of varying ligand-AuNPs is shown via stretching and compression of the elastic linker, respectively. High ligand-AuNP densities promote macrophage adhesion-regulated M2 polarization that inhibits M1 polarization. Remote switching of downward movement (“Lower Mag”) of ligand-AuNPs facilitates macrophage adhesion-regulated M2 polarization, which is conversely suppressed by their upward movement (“Upper Mag”), both in vitro and in vivo. These findings are consistent with human primary macrophages. These results provide fundamental understanding into designing materials with decorated nanostructures in both high ligand-AuNP density and downward movement of the ligand-AuNPs toward the substrate to stimulate adhesion-regulated M2 polarization of macrophages while suppressing pro-inflammatory M1 polarization, thereby facilitating tissue-healing responses.",

keywords = "elastic ligand movement, macrophage adhesion, macrophage polarization, magnetic switching, nanoparticle decoration",

author = "Ramar Thangam and Kim, {Myeong Soo} and Gunhyu Bae and Yuri Kim and Nayeon Kang and Sungkyu Lee and Jung, {Hee Joon} and Jinhyeok Jang and Hyojun Choi and Na Li and Minjin Kim and Sangwoo Park and Kim, {Seong Yeol} and Koo, {Thomas Myeongseok} and Fu, {Hong En} and Jeon, {Yoo Sang} and Andreja Ambriovi{\'c}-Ristov and Jae-Jun Song and Kim, {Soo Young} and Steve Park and Qiang Wei and Changhyun Ko and Lee, {Ki Bum} and Ramasamy Paulmurugan and Kim, {Young Keun} and Heemin Kang",

note = "Funding Information: R.T. and M.S.K. contributed equally to this work. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT) (No. 2020R1C1C1011038 and 2019R1A2C3006587). This work was also supported by a Korea University Grant. HAADF‐STEM imaging was conducted with the support of the Korea Basic Science Institute. This work made use of the EPIC facility of Northwestern University's NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS‐1542205), the MRSEC IRG2 program (NSF DMR‐1720139) at the Materials Research Center, the International Institute for Nanotechnology (IIN), the Keck Foundation, and the State of Illinois, through the IIN. The surgery was performed after obtaining approval from the Institutional Animal Care and Use Committee at Korea University for “KOREA‐2020‐0160” under the investigator of Prof. Jae‐Jun Song. Funding Information: R.T. and M.S.K. contributed equally to this work. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Science and ICT) (No. 2020R1C1C1011038 and 2019R1A2C3006587). This work was also supported by a Korea University Grant. HAADF-STEM imaging was conducted with the support of the Korea Basic Science Institute. This work made use of the EPIC facility of Northwestern University's NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the MRSEC IRG2 program (NSF DMR-1720139) at the Materials Research Center, the International Institute for Nanotechnology (IIN), the Keck Foundation, and the State of Illinois, through the IIN. The surgery was performed after obtaining approval from the Institutional Animal Care and Use Committee at Korea University for ?KOREA-2020-0160? under the investigator of Prof. Jae-Jun Song. Publisher Copyright: {\textcopyright} 2021 Wiley-VCH GmbH",

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AU - Thangam, Ramar

AU - Kim, Myeong Soo

AU - Bae, Gunhyu

AU - Kim, Yuri

AU - Kang, Nayeon

AU - Lee, Sungkyu

AU - Jung, Hee Joon

AU - Jang, Jinhyeok

AU - Choi, Hyojun

AU - Li, Na

AU - Kim, Minjin

AU - Park, Sangwoo

AU - Kim, Seong Yeol

AU - Koo, Thomas Myeongseok

AU - Fu, Hong En

AU - Jeon, Yoo Sang

AU - Ambriović-Ristov, Andreja

AU - Song, Jae-Jun

AU - Kim, Soo Young

AU - Park, Steve

AU - Wei, Qiang

AU - Ko, Changhyun

AU - Lee, Ki Bum

AU - Paulmurugan, Ramasamy

AU - Kim, Young Keun

AU - Kang, Heemin

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N2 - Design of materials with remote switchability of the movement of decorated nanostructures presenting cell-adhesive Arg-Gly-Asp ligand can decipher dynamic cell-material interactions in decorated ligand nanostructures. In this study, the decoration of ligand-bearing gold nanoparticles (ligand-AuNPs) on the magnetic nanoparticle (MNP) with varying ligand-AuNP densities is demonstrated, which are flexibly coupled to substrate in various MNP densities to maintain constant macroscopic ligand density. Magnetic switching of upward (“Upper Mag”) or downward (“Lower Mag”) movement of varying ligand-AuNPs is shown via stretching and compression of the elastic linker, respectively. High ligand-AuNP densities promote macrophage adhesion-regulated M2 polarization that inhibits M1 polarization. Remote switching of downward movement (“Lower Mag”) of ligand-AuNPs facilitates macrophage adhesion-regulated M2 polarization, which is conversely suppressed by their upward movement (“Upper Mag”), both in vitro and in vivo. These findings are consistent with human primary macrophages. These results provide fundamental understanding into designing materials with decorated nanostructures in both high ligand-AuNP density and downward movement of the ligand-AuNPs toward the substrate to stimulate adhesion-regulated M2 polarization of macrophages while suppressing pro-inflammatory M1 polarization, thereby facilitating tissue-healing responses.

AB - Design of materials with remote switchability of the movement of decorated nanostructures presenting cell-adhesive Arg-Gly-Asp ligand can decipher dynamic cell-material interactions in decorated ligand nanostructures. In this study, the decoration of ligand-bearing gold nanoparticles (ligand-AuNPs) on the magnetic nanoparticle (MNP) with varying ligand-AuNP densities is demonstrated, which are flexibly coupled to substrate in various MNP densities to maintain constant macroscopic ligand density. Magnetic switching of upward (“Upper Mag”) or downward (“Lower Mag”) movement of varying ligand-AuNPs is shown via stretching and compression of the elastic linker, respectively. High ligand-AuNP densities promote macrophage adhesion-regulated M2 polarization that inhibits M1 polarization. Remote switching of downward movement (“Lower Mag”) of ligand-AuNPs facilitates macrophage adhesion-regulated M2 polarization, which is conversely suppressed by their upward movement (“Upper Mag”), both in vitro and in vivo. These findings are consistent with human primary macrophages. These results provide fundamental understanding into designing materials with decorated nanostructures in both high ligand-AuNP density and downward movement of the ligand-AuNPs toward the substrate to stimulate adhesion-regulated M2 polarization of macrophages while suppressing pro-inflammatory M1 polarization, thereby facilitating tissue-healing responses.

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Remote Switching of Elastic Movement of Decorated Ligand Nanostructures Controls the Adhesion-Regulated Polarization of Host Macrophages

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Keywords

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