Repeated batch production of 1,3-propanediol from biodiesel derived waste glycerol by Klebsiella pneumoniae

Xiaoguang Yang, Dong Sup Kim, Han Suk Choi, Chan Kyum Kim, Laxmi Prasad Thapa, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)


In this study, 1,3-propanediol production was achieved from waste glycerol using Klebsiella pneumoniae ATCC 8724 under both batch and repeated batch fermentation. The inhibitory effects of the culture conditions were investigated considering the waste glycerol components. Anaerobic conditions showed better performance than aerobic conditions. Significant inhibitory effects in batch fermentation were observed in the following conditions: above pH 8, 60 g/L of initial glycerol concentration or 2% (w/v) of salts contents. However, improvement in 1,3-propanediol production and cell growth was represented below 1% (w/v) content of salts (NaCl and KCl). The highest yield of 1,3-propandiol using batch fermentation was achieved at 0.77 and 0.65 (mol/mol) with pure and waste glycerol, respectively. In addition, repeated batch fermentation was demonstrated with immobilized cells using waste glycerol. Compared to the first cycle of cultivation, 83% of 1,3-propanediol was obtained after five cycles of batch cultivation. The highest concentration and yield of 1,3-propanediol were respectively achieved 0.64 (mol/mol) and 20 g/L at the second cycle cultivation. The performance of by-products of the repeated batch fermentation with immobilized cells was also investigated for further application.

Original languageEnglish
Pages (from-to)660-669
Number of pages10
JournalChemical Engineering Journal
Publication statusPublished - 2017

Bibliographical note

Funding Information:
This research was supported by the ICT and Future Planning of Korea and the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) [grant number 2014R1A2A2A01007321]. This study was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education [grant number 2016R1D1A1A09918327] and Kwangwoon University (2017).

Publisher Copyright:
© 2016 Elsevier B.V.


  • 1,3-Propanediol
  • Immobilized cell
  • Inhibitory effect
  • Klebsiella pneumoniae
  • Waste glycerol

ASJC Scopus subject areas

  • General Chemistry
  • Environmental Chemistry
  • General Chemical Engineering
  • Industrial and Manufacturing Engineering


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