Repeated-batch production of omega-3 enriched biomass of Chlorella sorokiniana via calcium-induced homeoviscous adaptation

Sung Jin Shim, Min Eui Hong, Won Seok Chang, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


This study aimed to improve valuable omega-3 fatty acids production in freshwater microalgae at normal temperature by inducing homeoviscous adaptation using CaCl2, which could have a role in decreasing the cellular membrane fluidity followed by increasing the rigidity of cell wall and membranes. At 10 mM CaCl2, simultaneous biomass and lipid production was obtained by Ca2+-based single strategy without considerable sacrifice of cellular logarithmic growth in Chlorella sorokiniana. The cells cultured at 10 mM CaCl2 (1-stage) showed relatively high levels of cellular membrane fluidity, caused by increased content in unsaturated fatty acids, compared to the conventional culture strategy (2-stage). Moreover, when this process was recycled by repeated-batch fermentation, the EPA productivity of 1-stage was 4.338 mg L−1 d−1, conspicuously increased by over 1300% compared to 2-stage. This strategy enhances the valuable omega-3 production, which can be commercially used for mass cultivation of omega-3-enriched biomass in the microalgae industry.

Original languageEnglish
Article number122944
JournalBioresource technology
Publication statusPublished - 2020 May

Bibliographical note

Funding Information:
This study was supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) (grant number: 20172010202050 ).

Publisher Copyright:
© 2020 Elsevier Ltd


  • Ca-based 1-stage recycling strategy
  • Ca-induced homeoviscous adaptation
  • Chlorella sorokiniana
  • Eicosapentaenoic acid (EPA)
  • Omega-3 fatty acids

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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