Repeated production of hydrogen by sulfate re-addition in sulfur deprived culture of Chlamydomonas reinhardtii

Jun Pyo Kim, Kyoung Rok Kim, Seung Phill Choi, Se Jong Han, Mi Sun Kim, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


Biological hydrogen production by the green alga, Chlamydomonas reinhardtii can be induced in conditions of sulfur deprivation. In this study, we investigated the repeated and enhanced hydrogen production afforded by the re-addition of sulfate with monitoring of pH and concentration of chlorophyll and sulfate. Without adjustment of the pH, the optimal concentration of re-added sulfate was 30 μM for the hydrogen production. By the re-addition of 30 μM of sulfate and the adjustment of the pH during 4 cycles of repeated production, we obtained the maximum amount of 789 ml H2 l -1 culture, which is 3.4 times higher than that of one batch production without adjustment of pH, 236 ml H2 l-1 culture. This means that the enhancement of the hydrogen production can be achieved by the careful control of the sulfate re-addition and pH adjustment in the sulfur deprived culture.

Original languageEnglish
Pages (from-to)13387-13391
Number of pages5
JournalInternational Journal of Hydrogen Energy
Issue number24
Publication statusPublished - 2010 Dec
Externally publishedYes

Bibliographical note

Funding Information:
This Research was performed for the Hydrogen Energy R&D Center, one of the 21st Century Frontier R&D Programs, funded by the Ministry of Science and Technology of Korea.


  • Chlamydomonas reinhardtii
  • Green algae
  • Repeated hydrogen production
  • Sulfate re-addition
  • Sulfur deprivation
  • pH adjustment

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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