Resonance Raman Probes for Organelle-Specific Labeling in Live Cells

Andrey N. Kuzmin, Artem Pliss, Chang Keun Lim, Jeongyun Heo, Sehoon Kim, Alexander Rzhevskii, Bobo Gu, Ken Tye Yong, Shangchun Wen, Paras N. Prasad

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


Raman microspectroscopy provides for high-resolution non-invasive molecular analysis of biological samples and has a breakthrough potential for dissection of cellular molecular composition at a single organelle level. However, the potential of Raman microspectroscopy can be fully realized only when novel types of molecular probes distinguishable in the Raman spectroscopy modality are developed for labeling of specific cellular domains to guide spectrochemical spatial imaging. Here we report on the design of a next generation Raman probe, based on BlackBerry Quencher 650 compound, which provides unprecedentedly high signal intensity through the Resonance Raman (RR) enhancement mechanism. Remarkably, RR enhancement occurs with low-toxic red light, which is close to maximum transparency in the biological optical window. The utility of proposed RR probes was validated for targeting lysosomes in live cultured cells, which enabled identification and subsequent monitoring of dynamic changes in this organelle by Raman imaging.

Original languageEnglish
Article number28483
JournalScientific reports
Publication statusPublished - 2016 Jun 24

Bibliographical note

Funding Information:
This work at Buffalo (P. N. Prasad) was supported by the Air Force Office of Scientific Research grant FA9550-12-1-0226. The work at Changsha (S. Wen) was supported by the National Natural Science Foundation of China (Grant No. 11574079).

ASJC Scopus subject areas

  • General


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