Response surface optimised extraction and chromatographic purification of rosmarinic acid from Melissa officinalis leaves

Sooah Kim; Eun Ju Yun; Jin Seop Bak; Hojoung Lee; Sung Joon Lee; Cheong Tae Kim; Jong Hoon Lee; Kyoung Heon Kim

doi:10.1016/j.foodchem.2009.12.040

Response surface optimised extraction and chromatographic purification of rosmarinic acid from Melissa officinalis leaves

Sooah Kim, Eun Ju Yun, Jin Seop Bak, Hojoung Lee, Sung Joon Lee, Cheong Tae Kim, Jong Hoon Lee, Kyoung Heon Kim

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

The extraction of lemon balm (Melissa officinalis) leaves with aqueous methanol was optimised using response surface methodology. Fifteen runs were conducted following a Box-Behnken design (BBD) followed by ridge analysis using the concentration of methanol, the extraction temperature and time as the independent variables and taking the extraction yield of RA from lemon balm as the response variable. The optimal extraction conditions were a methanol concentration of 59.0% (v/v), a temperature of 54.8 °C and a time of 64.8 min, which gave a maximal RA yield of 46.1 mg RA/g dry materials. The RA extract was loaded onto a column packed with Sephadex LH-20 and then was eluted with 100% methanol, which resulted in RA with a purity of 38.8% and a yield of 43.8%. The purity of RA increased by 3.1-fold when compared to its initial purity in the extract obtained from extraction.

Original language	English
Pages (from-to)	521-526
Number of pages	6
Journal	Food Chemistry
Volume	121
Issue number	2
DOIs	https://doi.org/10.1016/j.foodchem.2009.12.040
Publication status	Published - 2010 Jul 15

Bibliographical note

Funding Information:
This study was supported by the Technology Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea (Grant No. 107100-03-3-SB020 ) and by a Grant from the BioGreen 21 Program, Rural Development Administration, Republic of Korea (Grant No. 20080401-034-049 ).

Keywords

Extraction
Lemon balm
Melissa officinalis
Purification
Response surface methodology
Rosmarinic acid

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2009.12.040

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title = "Response surface optimised extraction and chromatographic purification of rosmarinic acid from Melissa officinalis leaves",

abstract = "The extraction of lemon balm (Melissa officinalis) leaves with aqueous methanol was optimised using response surface methodology. Fifteen runs were conducted following a Box-Behnken design (BBD) followed by ridge analysis using the concentration of methanol, the extraction temperature and time as the independent variables and taking the extraction yield of RA from lemon balm as the response variable. The optimal extraction conditions were a methanol concentration of 59.0% (v/v), a temperature of 54.8 °C and a time of 64.8 min, which gave a maximal RA yield of 46.1 mg RA/g dry materials. The RA extract was loaded onto a column packed with Sephadex LH-20 and then was eluted with 100% methanol, which resulted in RA with a purity of 38.8% and a yield of 43.8%. The purity of RA increased by 3.1-fold when compared to its initial purity in the extract obtained from extraction.",

keywords = "Extraction, Lemon balm, Melissa officinalis, Purification, Response surface methodology, Rosmarinic acid",

author = "Sooah Kim and Yun, {Eun Ju} and Bak, {Jin Seop} and Hojoung Lee and Lee, {Sung Joon} and Kim, {Cheong Tae} and Lee, {Jong Hoon} and Kim, {Kyoung Heon}",

note = "Funding Information: This study was supported by the Technology Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea (Grant No. 107100-03-3-SB020 ) and by a Grant from the BioGreen 21 Program, Rural Development Administration, Republic of Korea (Grant No. 20080401-034-049 ). ",

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AU - Yun, Eun Ju

AU - Bak, Jin Seop

AU - Lee, Hojoung

AU - Lee, Sung Joon

AU - Kim, Cheong Tae

AU - Lee, Jong Hoon

AU - Kim, Kyoung Heon

N1 - Funding Information: This study was supported by the Technology Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea (Grant No. 107100-03-3-SB020 ) and by a Grant from the BioGreen 21 Program, Rural Development Administration, Republic of Korea (Grant No. 20080401-034-049 ).

PY - 2010/7/15

Y1 - 2010/7/15

N2 - The extraction of lemon balm (Melissa officinalis) leaves with aqueous methanol was optimised using response surface methodology. Fifteen runs were conducted following a Box-Behnken design (BBD) followed by ridge analysis using the concentration of methanol, the extraction temperature and time as the independent variables and taking the extraction yield of RA from lemon balm as the response variable. The optimal extraction conditions were a methanol concentration of 59.0% (v/v), a temperature of 54.8 °C and a time of 64.8 min, which gave a maximal RA yield of 46.1 mg RA/g dry materials. The RA extract was loaded onto a column packed with Sephadex LH-20 and then was eluted with 100% methanol, which resulted in RA with a purity of 38.8% and a yield of 43.8%. The purity of RA increased by 3.1-fold when compared to its initial purity in the extract obtained from extraction.

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Response surface optimised extraction and chromatographic purification of rosmarinic acid from Melissa officinalis leaves

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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