Response surface optimised extraction and chromatographic purification of rosmarinic acid from Melissa officinalis leaves

Sooah Kim, Eun Ju Yun, Jin Seop Bak, Hojoung Lee, Sung Joon Lee, Cheong Tae Kim, Jong Hoon Lee, Kyoung Heon Kim

    Research output: Contribution to journalArticlepeer-review

    48 Citations (Scopus)

    Abstract

    The extraction of lemon balm (Melissa officinalis) leaves with aqueous methanol was optimised using response surface methodology. Fifteen runs were conducted following a Box-Behnken design (BBD) followed by ridge analysis using the concentration of methanol, the extraction temperature and time as the independent variables and taking the extraction yield of RA from lemon balm as the response variable. The optimal extraction conditions were a methanol concentration of 59.0% (v/v), a temperature of 54.8 °C and a time of 64.8 min, which gave a maximal RA yield of 46.1 mg RA/g dry materials. The RA extract was loaded onto a column packed with Sephadex LH-20 and then was eluted with 100% methanol, which resulted in RA with a purity of 38.8% and a yield of 43.8%. The purity of RA increased by 3.1-fold when compared to its initial purity in the extract obtained from extraction.

    Original languageEnglish
    Pages (from-to)521-526
    Number of pages6
    JournalFood Chemistry
    Volume121
    Issue number2
    DOIs
    Publication statusPublished - 2010 Jul 15

    Bibliographical note

    Funding Information:
    This study was supported by the Technology Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea (Grant No. 107100-03-3-SB020 ) and by a Grant from the BioGreen 21 Program, Rural Development Administration, Republic of Korea (Grant No. 20080401-034-049 ).

    Keywords

    • Extraction
    • Lemon balm
    • Melissa officinalis
    • Purification
    • Response surface methodology
    • Rosmarinic acid

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Food Science

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