Restoration of miR-29b exerts anti-cancer effects on glioblastoma

Jaekyung Shin, Hyun Geun Shim, Taeyoung Hwang, Hyungsin Kim, Shin Hyuk Kang, Yun Sik Dho, Sung Hye Park, Sang Jeong Kim, Chul Kee Park

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


Background: Glioblastoma multiforme (GBM) is known as one of the most fatal forms of cancer. MicroRNAs have been widely implicated in the regulation of mammalian development and pathogenesis. The brain-enriched miR-29 subfamilies are known to be exclusively expressed in the developing brain, and they are aberrantly down-regulated in GBM. This study aims to elucidate the role of miR-29b in GBM development and the feasibility of therapeutic targeting using conjugated nanoparticles. Methods: After confirmation of miR-29b expression levels in GBM tissues by analysis of open source data, the anticancer effect of miR-29b was tested by the introduction of syn-hsa-miR-29b-3p in the A172 GBM cell line. In vitro studies of cell viability and apoptosis and ex vivo study using GBM tissue slice cultures from 3 patients and nanoparticle delivery of miR-29b were performed. Results: We discovered an increase in apoptotic cell populations with the introduction of miR-29b in the GBM cell line. An established human-derived GBM tissue slice culture system confirmed the anticancer effect of miR-29b-conjugated nanoparticles. Using PCR array, we found that exogenous miR-29b inhibits the expression of COL1A2, COL3A1, COL4A1, ELN, ITGA11, MMP24, and SPARC, which mediates an anticancer effect. Conclusions: miR-29b may serve as a putative therapeutic molecule when its expression is restored using a nanoparticle delivery system in GBM.

Original languageEnglish
Article number104
JournalCancer Cell International
Issue number1
Publication statusPublished - 2017 Nov 17
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Educa‑ tion (NRF‑2015R1D1A1A09057171) in Korea and the Seoul National University Hospital Research Fund (03‑2015‑0160).

Publisher Copyright:
© 2017 The Author(s).


  • Anti-cancer effect
  • Glioblastoma
  • MiR-29b
  • Nanoparticle

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Cancer Research


Dive into the research topics of 'Restoration of miR-29b exerts anti-cancer effects on glioblastoma'. Together they form a unique fingerprint.

Cite this