Retinoic acid modulates the level of nerve growth factor gene expression and proliferation of cultured human keratinocytes

Jin Woo Lee; Yong Kwon Lee; Bok Hwan Chun; Mu Hyoung Lee; Won Gi Bang; Young Woo Jin; Nahmhyun Chung

doi:10.1023/A:1021363203406

Retinoic acid modulates the level of nerve growth factor gene expression and proliferation of cultured human keratinocytes

Jin Woo Lee, Yong Kwon Lee, Bok Hwan Chun, Mu Hyoung Lee, Won Gi Bang, Young Woo Jin, Nahmhyun Chung

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

Abstract

Retinoic acid (RA) inhibited proliferation of cultured human keratinocytes. Southern blot analysis at 24 h showed that RA inhibited nerve growth factor (NGF) mRNA synthesis in a dose-dependent manner. However, RA stimulated the production of NGF protein up to 187% of control after 96 h and the treatment of cells with RA did not enhance apoptosis, either in the presence of low or high concentration of Ca²⁺, when compared to the control with DMSO.

Original language	English
Pages (from-to)	2035-2040
Number of pages	6
Journal	Biotechnology letters
Volume	24
Issue number	24
DOIs	https://doi.org/10.1023/A:1021363203406
Publication status	Published - 2002 Dec

Keywords

Apoptosis
Keratinocyte
Nerve growth factor
Proliferation
Retinoic acid

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1023/A:1021363203406

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title = "Retinoic acid modulates the level of nerve growth factor gene expression and proliferation of cultured human keratinocytes",

abstract = "Retinoic acid (RA) inhibited proliferation of cultured human keratinocytes. Southern blot analysis at 24 h showed that RA inhibited nerve growth factor (NGF) mRNA synthesis in a dose-dependent manner. However, RA stimulated the production of NGF protein up to 187% of control after 96 h and the treatment of cells with RA did not enhance apoptosis, either in the presence of low or high concentration of Ca2+, when compared to the control with DMSO.",

keywords = "Apoptosis, Keratinocyte, Nerve growth factor, Proliferation, Retinoic acid",

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AU - Lee, Jin Woo

AU - Lee, Yong Kwon

AU - Chun, Bok Hwan

AU - Lee, Mu Hyoung

AU - Bang, Won Gi

AU - Jin, Young Woo

AU - Chung, Nahmhyun

PY - 2002/12

Y1 - 2002/12

N2 - Retinoic acid (RA) inhibited proliferation of cultured human keratinocytes. Southern blot analysis at 24 h showed that RA inhibited nerve growth factor (NGF) mRNA synthesis in a dose-dependent manner. However, RA stimulated the production of NGF protein up to 187% of control after 96 h and the treatment of cells with RA did not enhance apoptosis, either in the presence of low or high concentration of Ca2+, when compared to the control with DMSO.

AB - Retinoic acid (RA) inhibited proliferation of cultured human keratinocytes. Southern blot analysis at 24 h showed that RA inhibited nerve growth factor (NGF) mRNA synthesis in a dose-dependent manner. However, RA stimulated the production of NGF protein up to 187% of control after 96 h and the treatment of cells with RA did not enhance apoptosis, either in the presence of low or high concentration of Ca2+, when compared to the control with DMSO.

KW - Apoptosis

KW - Keratinocyte

KW - Nerve growth factor

KW - Proliferation

KW - Retinoic acid

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Retinoic acid modulates the level of nerve growth factor gene expression and proliferation of cultured human keratinocytes

Abstract

Keywords

ASJC Scopus subject areas

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