Review of advances in glycan analysis on exosomes, cancer cells, and circulating cancer-derived glycoproteins with an emphasis on electrochemistry

Glycosylation, the intricate process of adding carbohydrate motifs to proteins, lipids, and exosomes on the cell surface, is crucial for both physiological and pathological mechanisms. Alterations in glycans significantly affect cancer cell metastasis by mediating cell-cell and cell-matrix interactions. The subtle changes in glycosylation during malignant transformations highlight the importance of analyzing cell and exosome surface glycosylation for prognostic and early treatment strategies in cancer. This review focuses on recent advancements in sensors for detecting surface glycans on cancer cells, exosomes, and circulating cancer-derived glycoproteins. Among various methods, electrochemical biosensors stand out as a promising tool, offering rapid and cost-effective glycan detection. These devices detect glycan interactions by measuring changes in electrical signals resulting from specific binding events. Techniques such as differential pulse voltammetry, impedance spectroscopy, and chrono amperometry are commonly employed for glycan detection using electrochemical biosensors. Researchers are exploring novel electrode materials and surface functionalization strategies to enhance sensor performance. Notably, selective binding probes such as lectins, aptamers, antibodies, and boronic acids are discussed, with lectins being the most prevalent for specific glycan analysis. By highlighting the significance of electrochemical techniques, emphasizing the role of selective binding probes, integrating microfluidics and miniaturized devices could lead to point-of-care applications for cancer diagnosis and monitoring. This review aims to provide valuable insights for researchers and clinicians working in the field of cancer glycomics.