Abstract
In our previous proteomic analysis, we isolated a small GTPase RabG3b as a salicylic acid-responsive protein in Arabidopsis (Oh et al. in Plant Cell 17:2832-2847, 2005). Here, we constructed transgenic plants overexpressing wild-type (RabG3bOX), constitutively active (RabG3bCA), and dominant negative (RabG3bDN) forms of RabG3b for functional studies. The phenotypes of these transgenic plants were indistinguishable from wild-type plants under normal growth conditions. However, both RabG3bOX and RabG3bCA plants displayed unrestricted hypersensitive programmed cell death against a fungal toxin Fumonisin B1 and a fungal pathogen Alternaria brassicicola, whereas no major difference between wildtype and RabG3bDN plants was observed. In addition, RabG3bOX and RabG3bCA plants underwent accelerated leaf senescence compared to wild-type and RabG3bDNplants. These results suggest that RabG3b is a modulator for cell death progression during pathogen response and senescence process in plants.
| Original language | English |
|---|---|
| Pages (from-to) | 79-87 |
| Number of pages | 9 |
| Journal | Journal of Plant Biology |
| Volume | 52 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 Apr |
Bibliographical note
Funding Information:Acknowledgments This work was supported by grants from the Plant Signaling Network Research Center (R11-2003-008-04004-0), the Biotechnology Development Program (2006-02762), and the Basic Science Program (R01-2007-000-10548-0) funded by the Korea Science and Engineering Foundation.
Keywords
- Alternaria brassicicola
- Fumonisin B1
- Hypersensitive response
- Leaf senescence
- Programmed cell death
- Rab GTPase
ASJC Scopus subject areas
- Plant Science