Role of phospholipase C-γ1 in insulin-like growth factor I-induced muscle differentiation of H9c2 cardiac myoblasts

Feng Hong, Keun ai Moon, Sam Soo Kim, Young Seol Kim, Young Kil Choi, Yun Soo Bae, Pann Ghill Suh, Sung Ho Ryu, Eui Ju Choi, Joohun Ha, Sung Soo Kim

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20 Citations (Scopus)


Insulin-like growth factor-I (IGF-I) regulates muscle differentiation through phosphatidylinositol 3-kinase (PI 3-kinase). Also it was recently reported that PI 3-kinase is involved in the activation of phospholipase C-γ1 (PLC-γ1). We investigated whether PLC-γ1 therefore plays a role in IGF-I-induced muscle differentiation using H9c2 rat cardiac myoblasts as a model. IGF-I was able to activate PLC-γ1 via both PI 3-kinase-dependent and tyrosine phosphorylation-dependent mechanisms in this model. However, PI 3-kinase appeared to play a more important role than tyrosine phosphorylation in IGF-I activation of PLC-γ1. In addition, PLC-γ1 activation was independent of Akt/protein kinase B (Akt/PKB). Importantly, PLC-γ1 was involved in IGF-I-induced muscle differentiation in parallel with Akt/PKB. Taken together, these results suggest that IGF-I regulation of muscle differentiation is dependent on the activation of PLC-γ1 and Akt/PKB, both of which are downstream mediators of PI 3-kinase.

Original languageEnglish
Pages (from-to)816-822
Number of pages7
JournalBiochemical and biophysical research communications
Issue number3
Publication statusPublished - 2001

Bibliographical note

Funding Information:
We are very grateful to Dr. Julian Downward at the Imperial Cancer Research Fund Laboratory, United Kingdom, and Dr. Masato Kasuga at Kobe University, Japan, for providing us with vectors encoding a constitutively active (p110*) and a dominant negative (Δp85) form of PI3-kinase. Dr. Feng Hong is a recipient of fellowship provided by Korea United Pharmaceutical Inc.

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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