SEPALLATA3 (SEP3) is important in determining flowering time as well as floral organ identity. although much is known about the regulation of floral organ identity by SEP3, its role as a downstream gene of FLOWERING LOCUS T (FT) for the regulation of ambient temperature-responsive flowering is poorly understood. here, we show that SEP3 as a downstream gene of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE3 (SPL3) and FT modulates the flowering time in response to different ambient temperatures. SEP3 overexpression showed temperature-insensitive flowering at 23°C and 16°C. this suggests that altered SEP3 activity affects ambient temperature-responsive flowering. however, a lesion in SEP3 did not obviously affect ambient temperature-responsive flowering. SEP3 expression was affected by altered SPL3 and FT activities in the leaf and shoot apical regions at different temperatures. these results suggest that the miR156-SPL3-FT circuitry directly or indirectly regulates SEP3 expression for the regulation of ambient temperature-responsive flowering in Arabidopsis.
Bibliographical noteFunding Information:
This work was supported by the Creative Research Initiatives of the National Research Foundation for the Ministry of Education, Science and Technology (R16–2008–106–01000–0 to J.H.A.) and by the Brain Korea 21 program (to J.H.L).
- Ambient temperature
- Flowering time
- miR156-SPL3-FT circuitry
ASJC Scopus subject areas
- Plant Science