Role of the methionine sulfoxide reductase MsrB3 in cold acclimation in Arabidopsis

Sun Jae Kwon; Soon Il Kwon; Min Seok Bae; Eun Ju Cho; Ohkmae K. Park

doi:10.1093/pcp/pcm143

Role of the methionine sulfoxide reductase MsrB3 in cold acclimation in Arabidopsis

Sun Jae Kwon, Soon Il Kwon, Min Seok Bae, Eun Ju Cho, Ohkmae K. Park

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

Methionine residues of proteins are a major target for oxidation by reactive oxygen species (ROS), which are generated in response to a variety of stress conditions. Methionine sulfoxide (MetO) reductases are present in most organisms and play protective roles in the cellular response to oxidative stress, reducing oxidized MetO back to Met. Previously, an Arabidopsis MetO reductase, MsrB3, was identified as a cold-responsive protein. Here we report that MsrB3 functions in the process of cold acclimation, thus contributing to cold tolerance. In contrast to normal, wild-type plants, msrb3 mutant plants lost the ability to become tolerant to freezing temperatures following cold pre-treatment. Furthermore, when exposed to low temperature, msrb3 plants exhibited a larger increase in MetO and H₂O₂ content and electrolyte leakage compared with wild-type and MsrB3 transgenic plants. It is also shown that MsrB3 is localized at the endoplasmic reticulum (ER). We propose that MsrB3 plays an important role in cold tolerance by eliminating MetO and ROS that accumulate at the ER during cold acclimation.

Original language	English
Pages (from-to)	1713-1723
Number of pages	11
Journal	Plant and Cell Physiology
Volume	48
Issue number	12
DOIs	https://doi.org/10.1093/pcp/pcm143
Publication status	Published - 2007 Dec

Bibliographical note

Funding Information:
We are grateful to Dr. Jong Bok Seo for technical assistance. MALDI-TOF MS analysis was conducted at Seoul Branch, Korea Basic Science Support Institute. We thank Dr. Inhwan Hwang for providing the BiP–GFP vector. This work was supported by grants from the Plant Signaling Network Research Center and the Crop Functional Genomics Center of the 21st Century Frontier Research Program funded by the Korea Science and Engineering Foundation, and the BioGreen 21 Program funded by the Rural Development Administration.

Keywords

Arabidopsis
Cold acclimation
Cold stress
Met sulfoxide reductase
Oxidative stress
Reactive oxygen species

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

Access to Document

10.1093/pcp/pcm143

Cite this

@article{2bd6ea3dd35546d58410c537dc89108a,

title = "Role of the methionine sulfoxide reductase MsrB3 in cold acclimation in Arabidopsis",

abstract = "Methionine residues of proteins are a major target for oxidation by reactive oxygen species (ROS), which are generated in response to a variety of stress conditions. Methionine sulfoxide (MetO) reductases are present in most organisms and play protective roles in the cellular response to oxidative stress, reducing oxidized MetO back to Met. Previously, an Arabidopsis MetO reductase, MsrB3, was identified as a cold-responsive protein. Here we report that MsrB3 functions in the process of cold acclimation, thus contributing to cold tolerance. In contrast to normal, wild-type plants, msrb3 mutant plants lost the ability to become tolerant to freezing temperatures following cold pre-treatment. Furthermore, when exposed to low temperature, msrb3 plants exhibited a larger increase in MetO and H2O2 content and electrolyte leakage compared with wild-type and MsrB3 transgenic plants. It is also shown that MsrB3 is localized at the endoplasmic reticulum (ER). We propose that MsrB3 plays an important role in cold tolerance by eliminating MetO and ROS that accumulate at the ER during cold acclimation.",

keywords = "Arabidopsis, Cold acclimation, Cold stress, Met sulfoxide reductase, Oxidative stress, Reactive oxygen species",

author = "Kwon, {Sun Jae} and Kwon, {Soon Il} and Bae, {Min Seok} and Cho, {Eun Ju} and Park, {Ohkmae K.}",

note = "Funding Information: We are grateful to Dr. Jong Bok Seo for technical assistance. MALDI-TOF MS analysis was conducted at Seoul Branch, Korea Basic Science Support Institute. We thank Dr. Inhwan Hwang for providing the BiP–GFP vector. This work was supported by grants from the Plant Signaling Network Research Center and the Crop Functional Genomics Center of the 21st Century Frontier Research Program funded by the Korea Science and Engineering Foundation, and the BioGreen 21 Program funded by the Rural Development Administration.",

year = "2007",

month = dec,

doi = "10.1093/pcp/pcm143",

language = "English",

volume = "48",

pages = "1713--1723",

journal = "Plant and Cell Physiology",

issn = "0032-0781",

publisher = "Oxford University Press",

number = "12",

}

TY - JOUR

T1 - Role of the methionine sulfoxide reductase MsrB3 in cold acclimation in Arabidopsis

AU - Kwon, Sun Jae

AU - Kwon, Soon Il

AU - Bae, Min Seok

AU - Cho, Eun Ju

AU - Park, Ohkmae K.

N1 - Funding Information: We are grateful to Dr. Jong Bok Seo for technical assistance. MALDI-TOF MS analysis was conducted at Seoul Branch, Korea Basic Science Support Institute. We thank Dr. Inhwan Hwang for providing the BiP–GFP vector. This work was supported by grants from the Plant Signaling Network Research Center and the Crop Functional Genomics Center of the 21st Century Frontier Research Program funded by the Korea Science and Engineering Foundation, and the BioGreen 21 Program funded by the Rural Development Administration.

PY - 2007/12

Y1 - 2007/12

N2 - Methionine residues of proteins are a major target for oxidation by reactive oxygen species (ROS), which are generated in response to a variety of stress conditions. Methionine sulfoxide (MetO) reductases are present in most organisms and play protective roles in the cellular response to oxidative stress, reducing oxidized MetO back to Met. Previously, an Arabidopsis MetO reductase, MsrB3, was identified as a cold-responsive protein. Here we report that MsrB3 functions in the process of cold acclimation, thus contributing to cold tolerance. In contrast to normal, wild-type plants, msrb3 mutant plants lost the ability to become tolerant to freezing temperatures following cold pre-treatment. Furthermore, when exposed to low temperature, msrb3 plants exhibited a larger increase in MetO and H2O2 content and electrolyte leakage compared with wild-type and MsrB3 transgenic plants. It is also shown that MsrB3 is localized at the endoplasmic reticulum (ER). We propose that MsrB3 plays an important role in cold tolerance by eliminating MetO and ROS that accumulate at the ER during cold acclimation.

AB - Methionine residues of proteins are a major target for oxidation by reactive oxygen species (ROS), which are generated in response to a variety of stress conditions. Methionine sulfoxide (MetO) reductases are present in most organisms and play protective roles in the cellular response to oxidative stress, reducing oxidized MetO back to Met. Previously, an Arabidopsis MetO reductase, MsrB3, was identified as a cold-responsive protein. Here we report that MsrB3 functions in the process of cold acclimation, thus contributing to cold tolerance. In contrast to normal, wild-type plants, msrb3 mutant plants lost the ability to become tolerant to freezing temperatures following cold pre-treatment. Furthermore, when exposed to low temperature, msrb3 plants exhibited a larger increase in MetO and H2O2 content and electrolyte leakage compared with wild-type and MsrB3 transgenic plants. It is also shown that MsrB3 is localized at the endoplasmic reticulum (ER). We propose that MsrB3 plays an important role in cold tolerance by eliminating MetO and ROS that accumulate at the ER during cold acclimation.

KW - Arabidopsis

KW - Cold acclimation

KW - Cold stress

KW - Met sulfoxide reductase

KW - Oxidative stress

KW - Reactive oxygen species

UR - http://www.scopus.com/inward/record.url?scp=37149026248&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcm143

DO - 10.1093/pcp/pcm143

M3 - Article

C2 - 17956860

AN - SCOPUS:37149026248

SN - 0032-0781

VL - 48

SP - 1713

EP - 1723

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

IS - 12

ER -

Role of the methionine sulfoxide reductase MsrB3 in cold acclimation in Arabidopsis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this