ROOT MERISTEM GROWTH FACTOR1 (RGF1)–RGF1 INSENSITIVE 1 peptide–receptor pair inhibits lateral root development via the MPK6–PUCHI module in Arabidopsis

Byeong Wook Jeon, Jin Sun Kim, Eunkyoo Oh, Na Young Kang, Jungmook Kim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

ROOT MERISTEM GROWTH FACTOR1 (RGF1) and its receptors RGF1 INSENSITIVEs (RGIs) regulate primary root meristem activity via a mitogen-activated protein kinase (MPK) signaling cascade in Arabidopsis. However, it is unknown how RGF1 regulates lateral root (LR) development. Here, we show that the RGF1–RGI1 peptide–receptor pair negatively regulates LR development via activation of PUCHI encoding AP2/EREBP. Exogenous RGF1 peptides inhibited LR development of the wild type. However, the rgi1 mutants were partially or fully insensitive to RGF1 during LR development, whereas four other rgi single mutants, namely rgi2, rgi3, rgi4, and rgi5, were sensitive to RGF1 in inhibiting LR formation. Consistent with this, the red fluorescent protein (RFP) signals driven by the RGF1 promoter were detected at stage I and the following stages, overlapping with RGI1 expression. PUCHI expression was significantly up-regulated by RGF1 but completely inhibited in rgi1. LR development of puchi1-1 was insensitive to RGF1. PUCHI expression driven by the RGI1 promoter reduced LR density in both the wild type and rgi1,2,3. Further, mpk6, but not mpk3, displayed significantly down-regulated PUCHI expression and insensitive LR development in response to RGF1. Collectively, these results suggest that the RGF1–RGI1 module negatively regulates LR development by activating PUCHI expression via MPK6.

Original languageEnglish
Pages (from-to)1475-1488
Number of pages14
JournalJournal of experimental botany
Volume74
Issue number5
DOIs
Publication statusPublished - 2023 Mar 13

Bibliographical note

Funding Information:
This research was supported by a grant from the Basic Research Lab Program (2020R1A42002901) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT.

Publisher Copyright:
© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved.

Keywords

  • Lateral root
  • leucine-rich repeat receptor kinase (LRR-RK)
  • MPK6
  • PUCHI
  • RGF1 INSENSITIVE 1 (RGI1)
  • ROOT MERISTEM GROWTH FACTOR 1 (RGF1)

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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