RUNX3 enhances TRAIL-induced apoptosis by upregulating DR5 in colorectal cancer

Bo Ram Kim; Seong Hye Park; Yoon A. Jeong; Yoo Jin Na; Jung Lim Kim; Min Jee Jo; Soyeon Jeong; Hye Kyeong Yun; Sang Cheul Oh; Dae Hee Lee

doi:10.1038/s41388-019-0693-x

RUNX3 enhances TRAIL-induced apoptosis by upregulating DR5 in colorectal cancer

Bo Ram Kim
, Seong Hye Park
, Yoon A. Jeong
, Yoo Jin Na
, Jung Lim Kim
, Min Jee Jo
, Soyeon Jeong
, Hye Kyeong Yun
, Sang Cheul Oh^*
, Dae Hee Lee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

RUNX3 is frequently inactivated by DNA hypermethylation in numerous cancers. Here, we show that RUNX3 has an important role in modulating apoptosis in immediate response to tumor necrosis factor-related apoptosis-including ligand (TRAIL). Importantly, no combined effect of TRAIL and RUNX3 was observed in non-cancerous cells. We investigated the expression of the death receptors (DRs) DR4 and DR5, which are related to TRAIL resistance. Overexpression of RUNX3 increased DR5 expression via induction of the reactive oxygen species (ROS)-endoplasmic reticulum (ER) stress-effector CHOP. Reduction of DR5 markedly decreased apoptosis enhanced by the combined therapy of TRAIL and RUNX3. Interestingly, RUNX3 induced reactive oxygen species production by inhibiting SOD3 transcription via binding to the Superoxide dismutase 3 (SOD3) promoter. Additionally, the combined effect of TRAIL and RUNX3 decreased tumor growth in xenograft models. Our results demonstrate a direct role for RUNX3 in TRAIL-induced apoptosis via activation of DR5 and provide further support for RUNX3 as an anti-tumor.

Original language	English
Pages (from-to)	3903-3918
Number of pages	16
Journal	Oncogene
Volume	38
Issue number	20
DOIs	https://doi.org/10.1038/s41388-019-0693-x
Publication status	Published - 2019 May 16

Bibliographical note

Funding Information:
Acknowledgements This work was supported by a National Research Foundation (NRF) of Korea grant funded the Korean government (MSIP) [NRF-2017R1A6A3A11030765] and was supported by Korea University Grant and was supported by project for cooperative R&D between Industry, Academy, and Research Institute funded Korea ministry of SMES and Startups in 2018 [Grants No. Co558375].

Publisher Copyright:
© 2019, Springer Nature Limited.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

Access to Document

10.1038/s41388-019-0693-x

Cite this

@article{20c7cb34d6ad4332a6374bd73d5814e0,

title = "RUNX3 enhances TRAIL-induced apoptosis by upregulating DR5 in colorectal cancer",

abstract = "RUNX3 is frequently inactivated by DNA hypermethylation in numerous cancers. Here, we show that RUNX3 has an important role in modulating apoptosis in immediate response to tumor necrosis factor-related apoptosis-including ligand (TRAIL). Importantly, no combined effect of TRAIL and RUNX3 was observed in non-cancerous cells. We investigated the expression of the death receptors (DRs) DR4 and DR5, which are related to TRAIL resistance. Overexpression of RUNX3 increased DR5 expression via induction of the reactive oxygen species (ROS)-endoplasmic reticulum (ER) stress-effector CHOP. Reduction of DR5 markedly decreased apoptosis enhanced by the combined therapy of TRAIL and RUNX3. Interestingly, RUNX3 induced reactive oxygen species production by inhibiting SOD3 transcription via binding to the Superoxide dismutase 3 (SOD3) promoter. Additionally, the combined effect of TRAIL and RUNX3 decreased tumor growth in xenograft models. Our results demonstrate a direct role for RUNX3 in TRAIL-induced apoptosis via activation of DR5 and provide further support for RUNX3 as an anti-tumor.",

author = "Kim, \{Bo Ram\} and Park, \{Seong Hye\} and Jeong, \{Yoon A.\} and Na, \{Yoo Jin\} and Kim, \{Jung Lim\} and Jo, \{Min Jee\} and Soyeon Jeong and Yun, \{Hye Kyeong\} and Oh, \{Sang Cheul\} and Lee, \{Dae Hee\}",

note = "Funding Information: Acknowledgements This work was supported by a National Research Foundation (NRF) of Korea grant funded the Korean government (MSIP) [NRF-2017R1A6A3A11030765] and was supported by Korea University Grant and was supported by project for cooperative R\&D between Industry, Academy, and Research Institute funded Korea ministry of SMES and Startups in 2018 [Grants No. Co558375]. Publisher Copyright: {\textcopyright} 2019, Springer Nature Limited.",

year = "2019",

month = may,

day = "16",

doi = "10.1038/s41388-019-0693-x",

language = "English",

volume = "38",

pages = "3903--3918",

journal = "Oncogene",

issn = "0950-9232",

publisher = "Springer Nature",

number = "20",

}

TY - JOUR

T1 - RUNX3 enhances TRAIL-induced apoptosis by upregulating DR5 in colorectal cancer

AU - Kim, Bo Ram

AU - Park, Seong Hye

AU - Jeong, Yoon A.

AU - Na, Yoo Jin

AU - Kim, Jung Lim

AU - Jo, Min Jee

AU - Jeong, Soyeon

AU - Yun, Hye Kyeong

AU - Oh, Sang Cheul

AU - Lee, Dae Hee

N1 - Funding Information: Acknowledgements This work was supported by a National Research Foundation (NRF) of Korea grant funded the Korean government (MSIP) [NRF-2017R1A6A3A11030765] and was supported by Korea University Grant and was supported by project for cooperative R&D between Industry, Academy, and Research Institute funded Korea ministry of SMES and Startups in 2018 [Grants No. Co558375]. Publisher Copyright: © 2019, Springer Nature Limited.

PY - 2019/5/16

Y1 - 2019/5/16

N2 - RUNX3 is frequently inactivated by DNA hypermethylation in numerous cancers. Here, we show that RUNX3 has an important role in modulating apoptosis in immediate response to tumor necrosis factor-related apoptosis-including ligand (TRAIL). Importantly, no combined effect of TRAIL and RUNX3 was observed in non-cancerous cells. We investigated the expression of the death receptors (DRs) DR4 and DR5, which are related to TRAIL resistance. Overexpression of RUNX3 increased DR5 expression via induction of the reactive oxygen species (ROS)-endoplasmic reticulum (ER) stress-effector CHOP. Reduction of DR5 markedly decreased apoptosis enhanced by the combined therapy of TRAIL and RUNX3. Interestingly, RUNX3 induced reactive oxygen species production by inhibiting SOD3 transcription via binding to the Superoxide dismutase 3 (SOD3) promoter. Additionally, the combined effect of TRAIL and RUNX3 decreased tumor growth in xenograft models. Our results demonstrate a direct role for RUNX3 in TRAIL-induced apoptosis via activation of DR5 and provide further support for RUNX3 as an anti-tumor.

AB - RUNX3 is frequently inactivated by DNA hypermethylation in numerous cancers. Here, we show that RUNX3 has an important role in modulating apoptosis in immediate response to tumor necrosis factor-related apoptosis-including ligand (TRAIL). Importantly, no combined effect of TRAIL and RUNX3 was observed in non-cancerous cells. We investigated the expression of the death receptors (DRs) DR4 and DR5, which are related to TRAIL resistance. Overexpression of RUNX3 increased DR5 expression via induction of the reactive oxygen species (ROS)-endoplasmic reticulum (ER) stress-effector CHOP. Reduction of DR5 markedly decreased apoptosis enhanced by the combined therapy of TRAIL and RUNX3. Interestingly, RUNX3 induced reactive oxygen species production by inhibiting SOD3 transcription via binding to the Superoxide dismutase 3 (SOD3) promoter. Additionally, the combined effect of TRAIL and RUNX3 decreased tumor growth in xenograft models. Our results demonstrate a direct role for RUNX3 in TRAIL-induced apoptosis via activation of DR5 and provide further support for RUNX3 as an anti-tumor.

UR - https://www.scopus.com/pages/publications/85060814910

U2 - 10.1038/s41388-019-0693-x

DO - 10.1038/s41388-019-0693-x

M3 - Article

C2 - 30692634

AN - SCOPUS:85060814910

SN - 0950-9232

VL - 38

SP - 3903

EP - 3918

JO - Oncogene

JF - Oncogene

IS - 20

ER -

RUNX3 enhances TRAIL-induced apoptosis by upregulating DR5 in colorectal cancer

Abstract

Bibliographical note

UN SDGs

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this