Runx3 inhibits IL-4 production in T cells via physical interaction with NFAT

Sung Ho Lee; Hyung Min Jeong; Jin Myung Choi; Young Chang Cho; Tae Sung Kim; Kwang Youl Lee; Bok Yun Kang

doi:10.1016/j.bbrc.2009.02.026

Runx3 inhibits IL-4 production in T cells via physical interaction with NFAT

Sung Ho Lee, Hyung Min Jeong, Jin Myung Choi, Young Chang Cho, Tae Sung Kim, Kwang Youl Lee, Bok Yun Kang

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Interleukin (IL)-4 plays a key role in T helper 2 (Th2) cell differentiation favoring humoral immune response. Regulation of IL-4 gene expression, therefore, is critically important for Th2 dependent responses and Th2 dominant disorders. In T cells, IL-4 gene expression is regulated positively or negatively by a combination of several transcription factors. Recently, enhanced IL-4 production was reported in Runx3 knockout mice; this implies negative regulation of IL-4 by Runx3. Runx proteins are transcription factors that have a Runt domain and have essential functions in development. In this study, the molecular mechanism that downregulates IL-4 expression was investigated. Runx3 inhibited IL-4 production in EL-4 T cells stimulated with PMA/ionomycin. Runx3-mediated IL-4 inhibition was NFAT-dependent, and Runx3 was physically associated with NFAT. Therefore, our results suggest that the interaction between NFAT and Runx3 is a mechanism that causes the negative regulation of IL-4, along with previously reported repression by T-bet.

Original language	English
Pages (from-to)	214-217
Number of pages	4
Journal	Biochemical and biophysical research communications
Volume	381
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2009.02.026
Publication status	Published - 2009 Apr 3

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2008-531-E00105) and Grant No. RTI05-01-01 from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE), Republic of Korea.

Keywords

IL-4
NFAT
Runx3

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2009.02.026

Cite this

@article{7b6e7abdba9747f997743b3f198f3e4c,

title = "Runx3 inhibits IL-4 production in T cells via physical interaction with NFAT",

abstract = "Interleukin (IL)-4 plays a key role in T helper 2 (Th2) cell differentiation favoring humoral immune response. Regulation of IL-4 gene expression, therefore, is critically important for Th2 dependent responses and Th2 dominant disorders. In T cells, IL-4 gene expression is regulated positively or negatively by a combination of several transcription factors. Recently, enhanced IL-4 production was reported in Runx3 knockout mice; this implies negative regulation of IL-4 by Runx3. Runx proteins are transcription factors that have a Runt domain and have essential functions in development. In this study, the molecular mechanism that downregulates IL-4 expression was investigated. Runx3 inhibited IL-4 production in EL-4 T cells stimulated with PMA/ionomycin. Runx3-mediated IL-4 inhibition was NFAT-dependent, and Runx3 was physically associated with NFAT. Therefore, our results suggest that the interaction between NFAT and Runx3 is a mechanism that causes the negative regulation of IL-4, along with previously reported repression by T-bet.",

keywords = "IL-4, NFAT, Runx3",

author = "Lee, {Sung Ho} and Jeong, {Hyung Min} and Choi, {Jin Myung} and Cho, {Young Chang} and Kim, {Tae Sung} and Lee, {Kwang Youl} and Kang, {Bok Yun}",

note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2008-531-E00105) and Grant No. RTI05-01-01 from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE), Republic of Korea.",

year = "2009",

month = apr,

day = "3",

doi = "10.1016/j.bbrc.2009.02.026",

language = "English",

volume = "381",

pages = "214--217",

journal = "Biochemical and biophysical research communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Runx3 inhibits IL-4 production in T cells via physical interaction with NFAT

AU - Lee, Sung Ho

AU - Jeong, Hyung Min

AU - Choi, Jin Myung

AU - Cho, Young Chang

AU - Kim, Tae Sung

AU - Lee, Kwang Youl

AU - Kang, Bok Yun

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2008-531-E00105) and Grant No. RTI05-01-01 from the Regional Technology Innovation Program of the Ministry of Commerce, Industry and Energy (MOCIE), Republic of Korea.

PY - 2009/4/3

Y1 - 2009/4/3

N2 - Interleukin (IL)-4 plays a key role in T helper 2 (Th2) cell differentiation favoring humoral immune response. Regulation of IL-4 gene expression, therefore, is critically important for Th2 dependent responses and Th2 dominant disorders. In T cells, IL-4 gene expression is regulated positively or negatively by a combination of several transcription factors. Recently, enhanced IL-4 production was reported in Runx3 knockout mice; this implies negative regulation of IL-4 by Runx3. Runx proteins are transcription factors that have a Runt domain and have essential functions in development. In this study, the molecular mechanism that downregulates IL-4 expression was investigated. Runx3 inhibited IL-4 production in EL-4 T cells stimulated with PMA/ionomycin. Runx3-mediated IL-4 inhibition was NFAT-dependent, and Runx3 was physically associated with NFAT. Therefore, our results suggest that the interaction between NFAT and Runx3 is a mechanism that causes the negative regulation of IL-4, along with previously reported repression by T-bet.

AB - Interleukin (IL)-4 plays a key role in T helper 2 (Th2) cell differentiation favoring humoral immune response. Regulation of IL-4 gene expression, therefore, is critically important for Th2 dependent responses and Th2 dominant disorders. In T cells, IL-4 gene expression is regulated positively or negatively by a combination of several transcription factors. Recently, enhanced IL-4 production was reported in Runx3 knockout mice; this implies negative regulation of IL-4 by Runx3. Runx proteins are transcription factors that have a Runt domain and have essential functions in development. In this study, the molecular mechanism that downregulates IL-4 expression was investigated. Runx3 inhibited IL-4 production in EL-4 T cells stimulated with PMA/ionomycin. Runx3-mediated IL-4 inhibition was NFAT-dependent, and Runx3 was physically associated with NFAT. Therefore, our results suggest that the interaction between NFAT and Runx3 is a mechanism that causes the negative regulation of IL-4, along with previously reported repression by T-bet.

KW - IL-4

KW - NFAT

KW - Runx3

UR - http://www.scopus.com/inward/record.url?scp=61849090317&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.02.026

DO - 10.1016/j.bbrc.2009.02.026

M3 - Article

C2 - 19338776

AN - SCOPUS:61849090317

SN - 0006-291X

VL - 381

SP - 214

EP - 217

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

IS - 2

ER -

Runx3 inhibits IL-4 production in T cells via physical interaction with NFAT

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this