S100ß, matrix metalloproteinase-9, D-dimer, and heat shock protein 70 are serologic biomarkers of acute cerebral infarction in a mouse model of transient MCA occlusion

Jong Il Choi, Sung Kon Ha, Dong Jun Lim, Sang Dae Kim, Se Hoon Kim

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    19 Citations (Scopus)

    Abstract

    Objective: Diagnosing acute cerebral infarction is crucial in determining prognosis of stroke patients. Although many serologic tests for prompt diagnosis are available, the clinical application of serologic tests is currently limited. We investigated whether S100β, matrix metalloproteinase-9 (MMP-9), D-dimer, and heat shock protein 70 (HSP70) can be used as biomarkers for acute cerebral infarction. Methods: Focal cerebral ischemia was induced using the modified intraluminal filament technique. Mice were randomly assigned to 30-minute occlusion (n=10), 60-minute occlusion (n=10), or sham (n=5) groups. Four hours later, neurological deficits were evaluated and blood samples were obtained. Infarction volumes were calculated and plasma S100β, MMP-9, D-dimer, and HSP70 levels were measured using enzyme-linked immunosorbent assay. Results: The average infarction volume was 12.32±2.31 mm3 and 46.9±7.43 mm3 in the 30-and 60-minute groups, respectively. The mean neurological score in the two ischemic groups was 1.6±0.55 and 3.2±0.70, respectively. S100β, MMP-9, and HSP70 expressions significantly increased after 4 hours of ischemia (p=0.001). Furthermore, S100β and MMP-9 expressions correlated with infarction volumes (p<0.001) and neurological deficits (p<0.001). There was no significant difference in D-dimer expression between groups (p=0.843). The area under the receiver operating characteristic curve (AUC) showed high sensitivity and specificity for MMP-9, HSP70 (AUC=1), and S100β (AUC=0.98). Conclusion: S100β, MMP-9, and HSP70 can complement current diagnostic tools to assess cerebral infarction, suggesting their use as potential biomarkers for acute cerebral infarction.

    Original languageEnglish
    Pages (from-to)548-558
    Number of pages11
    JournalJournal of Korean Neurosurgical Society
    Volume61
    Issue number5
    DOIs
    Publication statusPublished - 2018 Sept

    Bibliographical note

    Publisher Copyright:
    © 2018 The Korean Neurosurgical Society.

    Keywords

    • Acute ischemic stroke
    • Biomarkers
    • Cerebral infarction volume

    ASJC Scopus subject areas

    • Surgery
    • General Neuroscience
    • Clinical Neurology

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