Sample preparation method for plasma membrane proteome analysis

Seung Ah Park; Mi Ryung Kim; Pan Kyeom Kim; Hyung Don Cho; Gi Yeon Han; Chan Wha Kim

doi:10.1016/j.jchromb.2008.07.028

Sample preparation method for plasma membrane proteome analysis

Seung Ah Park, Mi Ryung Kim, Pan Kyeom Kim, Hyung Don Cho, Gi Yeon Han, Chan Wha Kim

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.

Original language	English
Pages (from-to)	177-180
Number of pages	4
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	872
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2008.07.028
Publication status	Published - 2008 Sept 1

Bibliographical note

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

Percoll
Plasma membrane
Two-dimensional electrophoresis
Two-phase extraction
Ultracentrifugation
hMSCs

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

Access to Document

10.1016/j.jchromb.2008.07.028

Cite this

@article{9a7bbe21094e4100b3c8a40e954168e1,

title = "Sample preparation method for plasma membrane proteome analysis",

abstract = "The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.",

keywords = "Percoll, Plasma membrane, Two-dimensional electrophoresis, Two-phase extraction, Ultracentrifugation, hMSCs",

author = "Park, {Seung Ah} and Kim, {Mi Ryung} and Kim, {Pan Kyeom} and Cho, {Hyung Don} and Han, {Gi Yeon} and Kim, {Chan Wha}",

year = "2008",

month = sep,

day = "1",

doi = "10.1016/j.jchromb.2008.07.028",

language = "English",

volume = "872",

pages = "177--180",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Sample preparation method for plasma membrane proteome analysis

AU - Park, Seung Ah

AU - Kim, Mi Ryung

AU - Kim, Pan Kyeom

AU - Cho, Hyung Don

AU - Han, Gi Yeon

AU - Kim, Chan Wha

PY - 2008/9/1

Y1 - 2008/9/1

N2 - The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.

AB - The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.

KW - Percoll

KW - Plasma membrane

KW - Two-dimensional electrophoresis

KW - Two-phase extraction

KW - Ultracentrifugation

KW - hMSCs

UR - http://www.scopus.com/inward/record.url?scp=49849096458&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49849096458&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2008.07.028

DO - 10.1016/j.jchromb.2008.07.028

M3 - Article

C2 - 18692447

AN - SCOPUS:49849096458

SN - 1570-0232

VL - 872

SP - 177

EP - 180

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 1-2

ER -

Sample preparation method for plasma membrane proteome analysis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this