SAP couples Fyn to SLAM immune receptors

Betty Chan; Arpad Lanyi; Hyun Kyu Song; Jan Griesbach; Maria Simarro-Grande; Florence Poy; Duncan Howie; Janos Sumegi; Cox Terhorst; Michael J. Eck

doi:10.1038/ncb920

SAP couples Fyn to SLAM immune receptors

Betty Chan, Arpad Lanyi, Hyun Kyu Song, Jan Griesbach, Maria Simarro-Grande, Florence Poy, Duncan Howie, Janos Sumegi, Cox Terhorst, Michael J. Eck

Research output: Contribution to journal › Article › peer-review

243 Citations (Scopus)

Abstract

SAP (SLAM-associated protein) is a small lymphocyte-specific signalling molecule that is defective or absent in patients with X-linked lymphoproliferative syndrome (XLP)^1-3. Consistent with its single src homology 2 (SH2) domain architecture and unusually high affinity for SLAM (also called CD150), SAP has been suggested to function by blocking binding of SHP-2 or other SH2-containing signalling proteins to SLAM receptors^1,4. Additionally, SAP has recently been shown to be required for recruitment and activation of the Src-family kinase FynT after SLAM ligation⁵. This signalling 'adaptor' function has been difficult to conceptualize, because unlike typical SH2-adaptor proteins. SAP contains only a single SH2 domain and lacks other recognized protein interaction domains or motifs. Here, we show that the SAP SH2 domain blinds to the SH3 domain of FynT and directly couple FynT to SLAM. The crystal structure of a ternary SLAM-SAP-Fyn-SH3 complex reveals that SAP binds the FynT SH3 domain through a surface-surface interaction that does not involve canonical SH3 or SH2 binding in teractions. The observed mode of binding to the Fyn-SH3 domain is expected to preclude the auto-inhibited conformation of Fyn, thereby promoting activation of the kinase after recruitment. These findings broaden our understanding of the functional repertoire of SH3 and SH2 domains.

Original language	English
Pages (from-to)	155-160
Number of pages	6
Journal	Nature Cell Biology
Volume	5
Issue number	2
DOIs	https://doi.org/10.1038/ncb920
Publication status	Published - 2003 Feb 1
Externally published	Yes

Bibliographical note

Funding Information:
ACKNOWLEDGEMENTS We thank S.J. Freedman for assistance with the ITC experiments and helpful discussions. This work was supported in part by grants from the National Institutes of Health (M.J.E. and C.T.) and the Hungarian Academy of Sciences (A.L.). M.J.E. is a recipient of the Leukaemia and Lymphoma Society Scholar Award. Correspondence and requests for materials should be addressed to M.J.E.

ASJC Scopus subject areas

Cell Biology

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title = "SAP couples Fyn to SLAM immune receptors",

abstract = "SAP (SLAM-associated protein) is a small lymphocyte-specific signalling molecule that is defective or absent in patients with X-linked lymphoproliferative syndrome (XLP)1-3. Consistent with its single src homology 2 (SH2) domain architecture and unusually high affinity for SLAM (also called CD150), SAP has been suggested to function by blocking binding of SHP-2 or other SH2-containing signalling proteins to SLAM receptors1,4. Additionally, SAP has recently been shown to be required for recruitment and activation of the Src-family kinase FynT after SLAM ligation5. This signalling 'adaptor' function has been difficult to conceptualize, because unlike typical SH2-adaptor proteins. SAP contains only a single SH2 domain and lacks other recognized protein interaction domains or motifs. Here, we show that the SAP SH2 domain blinds to the SH3 domain of FynT and directly couple FynT to SLAM. The crystal structure of a ternary SLAM-SAP-Fyn-SH3 complex reveals that SAP binds the FynT SH3 domain through a surface-surface interaction that does not involve canonical SH3 or SH2 binding in teractions. The observed mode of binding to the Fyn-SH3 domain is expected to preclude the auto-inhibited conformation of Fyn, thereby promoting activation of the kinase after recruitment. These findings broaden our understanding of the functional repertoire of SH3 and SH2 domains.",

author = "Betty Chan and Arpad Lanyi and Song, {Hyun Kyu} and Jan Griesbach and Maria Simarro-Grande and Florence Poy and Duncan Howie and Janos Sumegi and Cox Terhorst and Eck, {Michael J.}",

note = "Funding Information: ACKNOWLEDGEMENTS We thank S.J. Freedman for assistance with the ITC experiments and helpful discussions. This work was supported in part by grants from the National Institutes of Health (M.J.E. and C.T.) and the Hungarian Academy of Sciences (A.L.). M.J.E. is a recipient of the Leukaemia and Lymphoma Society Scholar Award. Correspondence and requests for materials should be addressed to M.J.E.",

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AU - Lanyi, Arpad

AU - Song, Hyun Kyu

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AU - Poy, Florence

AU - Howie, Duncan

AU - Sumegi, Janos

AU - Terhorst, Cox

AU - Eck, Michael J.

N1 - Funding Information: ACKNOWLEDGEMENTS We thank S.J. Freedman for assistance with the ITC experiments and helpful discussions. This work was supported in part by grants from the National Institutes of Health (M.J.E. and C.T.) and the Hungarian Academy of Sciences (A.L.). M.J.E. is a recipient of the Leukaemia and Lymphoma Society Scholar Award. Correspondence and requests for materials should be addressed to M.J.E.

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N2 - SAP (SLAM-associated protein) is a small lymphocyte-specific signalling molecule that is defective or absent in patients with X-linked lymphoproliferative syndrome (XLP)1-3. Consistent with its single src homology 2 (SH2) domain architecture and unusually high affinity for SLAM (also called CD150), SAP has been suggested to function by blocking binding of SHP-2 or other SH2-containing signalling proteins to SLAM receptors1,4. Additionally, SAP has recently been shown to be required for recruitment and activation of the Src-family kinase FynT after SLAM ligation5. This signalling 'adaptor' function has been difficult to conceptualize, because unlike typical SH2-adaptor proteins. SAP contains only a single SH2 domain and lacks other recognized protein interaction domains or motifs. Here, we show that the SAP SH2 domain blinds to the SH3 domain of FynT and directly couple FynT to SLAM. The crystal structure of a ternary SLAM-SAP-Fyn-SH3 complex reveals that SAP binds the FynT SH3 domain through a surface-surface interaction that does not involve canonical SH3 or SH2 binding in teractions. The observed mode of binding to the Fyn-SH3 domain is expected to preclude the auto-inhibited conformation of Fyn, thereby promoting activation of the kinase after recruitment. These findings broaden our understanding of the functional repertoire of SH3 and SH2 domains.

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SAP couples Fyn to SLAM immune receptors

Abstract

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