Treatment of helper T (Th) cells with saponins from soy bean and mung bean prevented their activation by inhibiting cell proliferation and cytokine secretion. However, the saponins did not affect the expression of major histocompatibility complex class II (Ab) and co-stimulatory molecule (CD86) on professional antigen-presenting cells. Instead, the saponins directly inhibited Th cell proliferation by blocking the G1 to S phase cell cycle transition. Moreover, blocking of the cell cycle by the saponins was achieved by decreased expression of cyclin D1 and cyclin E, and constitutive expression of p27KIP1. Saponins also increased stability of p27KIP1 in Th cells after antigenic stimulation.
Bibliographical noteFunding Information:
Acknowledgments This research was supported by Technology Development Program for Food (109141-03), and the Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. This work was also supported by Next-Generation BioGreen 21 program (No. PJ008089), Rural Development Administration, Republic of Korea.
- Cell cycle
- Helper T cell
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology