Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. golden tower)

S. J. Kim, S. J. Lee, B. D. Kim, K. H. Paek

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49 Citations (Scopus)

Abstract

We previously established a system of in vitro regeneration and Agrobacterium-mediated transformation for hot pepper plants. The level of protection against cucumber mosaic virus in the progeny of the transgenic hot pepper plants that express cucumber mosaic virus (CMV) satellite RNA was investigated. The transgenic hot pepper plants were self-fertilized, and their progeny were tested for stable inheritance and expression of the cDNA of CMV satellite RNA. Polymerase chain reaction and RNA gel blot analyses showed that the introduced gene was stably transmitted and expressed in the progeny. Symptom attenuation in the offspring was confirmed upon inoculation with CMV-Y or CMV-Korea (CMV-Kor) strains.

Original languageEnglish
Pages (from-to)825-830
Number of pages6
JournalPlant Cell Reports
Volume16
Issue number12
DOIs
Publication statusPublished - 1997

Bibliographical note

Funding Information:
Acknowledgements This study was supported in part by grants from the Korean Science and Engineering Foundation through the Research Center for the Development of Advanced Horticultural Technology, the Korean Ministry of Science and Technology through the HAN project, and the Korean Research Foundation through the Non-Directed Research Fund.

Keywords

  • Cucumber mosaic virus
  • Local lesion assay
  • Satellite-RNA-mediated protection
  • Transgenic hot pepper plants

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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