Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. golden tower)

S. J. Kim; S. J. Lee; B. D. Kim; K. H. Paek

doi:10.1007/s002990050328

Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. golden tower)

S. J. Kim, S. J. Lee, B. D. Kim, K. H. Paek

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

We previously established a system of in vitro regeneration and Agrobacterium-mediated transformation for hot pepper plants. The level of protection against cucumber mosaic virus in the progeny of the transgenic hot pepper plants that express cucumber mosaic virus (CMV) satellite RNA was investigated. The transgenic hot pepper plants were self-fertilized, and their progeny were tested for stable inheritance and expression of the cDNA of CMV satellite RNA. Polymerase chain reaction and RNA gel blot analyses showed that the introduced gene was stably transmitted and expressed in the progeny. Symptom attenuation in the offspring was confirmed upon inoculation with CMV-Y or CMV-Korea (CMV-Kor) strains.

Original language	English
Pages (from-to)	825-830
Number of pages	6
Journal	Plant Cell Reports
Volume	16
Issue number	12
DOIs	https://doi.org/10.1007/s002990050328
Publication status	Published - 1997

Keywords

Cucumber mosaic virus
Local lesion assay
Satellite-RNA-mediated protection
Transgenic hot pepper plants

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s002990050328

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title = "Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. golden tower)",

abstract = "We previously established a system of in vitro regeneration and Agrobacterium-mediated transformation for hot pepper plants. The level of protection against cucumber mosaic virus in the progeny of the transgenic hot pepper plants that express cucumber mosaic virus (CMV) satellite RNA was investigated. The transgenic hot pepper plants were self-fertilized, and their progeny were tested for stable inheritance and expression of the cDNA of CMV satellite RNA. Polymerase chain reaction and RNA gel blot analyses showed that the introduced gene was stably transmitted and expressed in the progeny. Symptom attenuation in the offspring was confirmed upon inoculation with CMV-Y or CMV-Korea (CMV-Kor) strains.",

keywords = "Cucumber mosaic virus, Local lesion assay, Satellite-RNA-mediated protection, Transgenic hot pepper plants",

author = "Kim, {S. J.} and Lee, {S. J.} and Kim, {B. D.} and Paek, {K. H.}",

note = "Funding Information: Acknowledgements This study was supported in part by grants from the Korean Science and Engineering Foundation through the Research Center for the Development of Advanced Horticultural Technology, the Korean Ministry of Science and Technology through the HAN project, and the Korean Research Foundation through the Non-Directed Research Fund.",

year = "1997",

doi = "10.1007/s002990050328",

language = "English",

volume = "16",

pages = "825--830",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Verlag",

number = "12",

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TY - JOUR

T1 - Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. golden tower)

AU - Kim, S. J.

AU - Lee, S. J.

AU - Kim, B. D.

AU - Paek, K. H.

N1 - Funding Information: Acknowledgements This study was supported in part by grants from the Korean Science and Engineering Foundation through the Research Center for the Development of Advanced Horticultural Technology, the Korean Ministry of Science and Technology through the HAN project, and the Korean Research Foundation through the Non-Directed Research Fund.

PY - 1997

Y1 - 1997

N2 - We previously established a system of in vitro regeneration and Agrobacterium-mediated transformation for hot pepper plants. The level of protection against cucumber mosaic virus in the progeny of the transgenic hot pepper plants that express cucumber mosaic virus (CMV) satellite RNA was investigated. The transgenic hot pepper plants were self-fertilized, and their progeny were tested for stable inheritance and expression of the cDNA of CMV satellite RNA. Polymerase chain reaction and RNA gel blot analyses showed that the introduced gene was stably transmitted and expressed in the progeny. Symptom attenuation in the offspring was confirmed upon inoculation with CMV-Y or CMV-Korea (CMV-Kor) strains.

AB - We previously established a system of in vitro regeneration and Agrobacterium-mediated transformation for hot pepper plants. The level of protection against cucumber mosaic virus in the progeny of the transgenic hot pepper plants that express cucumber mosaic virus (CMV) satellite RNA was investigated. The transgenic hot pepper plants were self-fertilized, and their progeny were tested for stable inheritance and expression of the cDNA of CMV satellite RNA. Polymerase chain reaction and RNA gel blot analyses showed that the introduced gene was stably transmitted and expressed in the progeny. Symptom attenuation in the offspring was confirmed upon inoculation with CMV-Y or CMV-Korea (CMV-Kor) strains.

KW - Cucumber mosaic virus

KW - Local lesion assay

KW - Satellite-RNA-mediated protection

KW - Transgenic hot pepper plants

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DO - 10.1007/s002990050328

M3 - Article

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SN - 0721-7714

VL - 16

SP - 825

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JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 12

ER -

Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. golden tower)

Abstract

Keywords

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