Abstract
We previously established a system of in vitro regeneration and Agrobacterium-mediated transformation for hot pepper plants. The level of protection against cucumber mosaic virus in the progeny of the transgenic hot pepper plants that express cucumber mosaic virus (CMV) satellite RNA was investigated. The transgenic hot pepper plants were self-fertilized, and their progeny were tested for stable inheritance and expression of the cDNA of CMV satellite RNA. Polymerase chain reaction and RNA gel blot analyses showed that the introduced gene was stably transmitted and expressed in the progeny. Symptom attenuation in the offspring was confirmed upon inoculation with CMV-Y or CMV-Korea (CMV-Kor) strains.
Original language | English |
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Pages (from-to) | 825-830 |
Number of pages | 6 |
Journal | Plant Cell Reports |
Volume | 16 |
Issue number | 12 |
DOIs | |
Publication status | Published - 1997 |
Bibliographical note
Funding Information:Acknowledgements This study was supported in part by grants from the Korean Science and Engineering Foundation through the Research Center for the Development of Advanced Horticultural Technology, the Korean Ministry of Science and Technology through the HAN project, and the Korean Research Foundation through the Non-Directed Research Fund.
Keywords
- Cucumber mosaic virus
- Local lesion assay
- Satellite-RNA-mediated protection
- Transgenic hot pepper plants
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science