Sauchinone reduces oxygen-glucose deprivation-evoked neuronal cell death via suppression of intracellular radical production

In Young Choi, Hua Yan, Yong Ki Park, Won Ki Kim

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Sauchinone, a biologically active lignan isolated from Saururus chinensis, has been reported to show cytoprotective, anti-inflammatory and anti-apoptotic effects. However, little study has been done of the anti-ischemic/hypoxic effect of sauchinone. The present study investigates the anti-ischemic/hypoxic effect of sauchinone by using ischemia/hypoxia-sensitive neuronal cells. We found that sauchinone significantly prevented cortical neurons from oxygen-glucose deprivation (OGD) followed by re-oxygenation. Sauchinone did not inhibit both NMDA-induced cell membrane depolarization and intracellular calcium influx. We further found that sauchinone cannot directly scavenge reactive oxygen and nitrogen species such as H2O2 and peroxynitrite. Sauchinone, however, greatly reduced the formation of reactive oxygen and nitrogen species in neurons exposed to OGD/reoxygenation and inhibited the depolarization of mitochondrial transmembrane potential induced by OGD/reoxygenation. In accordance with diminishment of endogenous ROS production, sauchinone restored the decreased activities of antioxidant enzymes catalase and SOD evoked by OGD/reoxygenation. Specifically, sauchinone up-regulated the activity of catalase, indicating that sauchinone could be a useful cytoprotectant.

    Original languageEnglish
    Pages (from-to)1599-1606
    Number of pages8
    JournalArchives of pharmacal research
    Volume32
    Issue number11
    DOIs
    Publication statusPublished - 2009 Nov

    Bibliographical note

    Funding Information:
    This study was supported by a grant (#2009K001250) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, a grant from the Brain Korea 21 Project (to Dr. I. Y. Choi), and a grant from the Oriental Medicine R&D Project (B080027) of the Ministry for Health, Welfare and Family Affairs, Republic of Korea.

    Keywords

    • Catalase
    • Cortical neuron
    • Oxidative stress
    • Oxygen-glucose deprivation
    • Sauchinone

    ASJC Scopus subject areas

    • Molecular Medicine
    • Drug Discovery
    • Organic Chemistry

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