SB203580 is a p38 MAPK inhibitor that has been implicated in the activation of c-Raf. This study shows that the addition of SB203580 to PC12 cells causes the sustained activation of B-Raf but not of ERK. The addition of SB203580 prolonged the transient activation of both B-Raf and ERK by EGF alone. No significant change was detected in MAPKAPK-2 activity at low concentrations of SB203580, which induced neurite outgrowth in the EGF-stimulated PC12 cells. Therefore, these results indicate that SB203580 influences not only c-Raf as previously reported, but can also induce the activation of B-Raf, which in conjunction with EGF causes the sustained activation of ERK and differentiation in PC12 cells.
|Number of pages
|Published - 2004 Jul
Bibliographical noteFunding Information:
This work was supported in part by a grant from the International Cooperation Research Program (grant No. 2U02840) and Systems Biology Research Program (grant No. 2N26170) of the Ministry of Science and Technology.
- Neurite outgrowth
- Nuclear translocation
- PC12 cells
ASJC Scopus subject areas