SB203580 induces prolonged B-Raf activation and promotes neuronal differentiation upon EGF treatment of PC12 cells

Seunghee Yoon, Rony Seger, Eui Ju Choi, Young Sook Yoo

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    SB203580 is a p38 MAPK inhibitor that has been implicated in the activation of c-Raf. This study shows that the addition of SB203580 to PC12 cells causes the sustained activation of B-Raf but not of ERK. The addition of SB203580 prolonged the transient activation of both B-Raf and ERK by EGF alone. No significant change was detected in MAPKAPK-2 activity at low concentrations of SB203580, which induced neurite outgrowth in the EGF-stimulated PC12 cells. Therefore, these results indicate that SB203580 influences not only c-Raf as previously reported, but can also induce the activation of B-Raf, which in conjunction with EGF causes the sustained activation of ERK and differentiation in PC12 cells.

    Original languageEnglish
    Pages (from-to)799-805
    Number of pages7
    JournalBiochemistry (Moscow)
    Volume69
    Issue number7
    DOIs
    Publication statusPublished - 2004 Jul

    Bibliographical note

    Funding Information:
    This work was supported in part by a grant from the International Cooperation Research Program (grant No. 2U02840) and Systems Biology Research Program (grant No. 2N26170) of the Ministry of Science and Technology.

    Keywords

    • B-Raf
    • ERK
    • Neurite outgrowth
    • Nuclear translocation
    • PC12 cells
    • SB203580

    ASJC Scopus subject areas

    • Biochemistry

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