SB203580 induces prolonged B-Raf activation and promotes neuronal differentiation upon EGF treatment of PC12 cells

Seunghee Yoon; Rony Seger; Eui Ju Choi; Young Sook Yoo

doi:10.1023/B:BIRY.0000040206.71415.6f

SB203580 induces prolonged B-Raf activation and promotes neuronal differentiation upon EGF treatment of PC12 cells

Seunghee Yoon, Rony Seger, Eui Ju Choi, Young Sook Yoo

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

SB203580 is a p38 MAPK inhibitor that has been implicated in the activation of c-Raf. This study shows that the addition of SB203580 to PC12 cells causes the sustained activation of B-Raf but not of ERK. The addition of SB203580 prolonged the transient activation of both B-Raf and ERK by EGF alone. No significant change was detected in MAPKAPK-2 activity at low concentrations of SB203580, which induced neurite outgrowth in the EGF-stimulated PC12 cells. Therefore, these results indicate that SB203580 influences not only c-Raf as previously reported, but can also induce the activation of B-Raf, which in conjunction with EGF causes the sustained activation of ERK and differentiation in PC12 cells.

Original language	English
Pages (from-to)	799-805
Number of pages	7
Journal	Biochemistry (Moscow)
Volume	69
Issue number	7
DOIs	https://doi.org/10.1023/B:BIRY.0000040206.71415.6f
Publication status	Published - 2004 Jul

Bibliographical note

Funding Information:
This work was supported in part by a grant from the International Cooperation Research Program (grant No. 2U02840) and Systems Biology Research Program (grant No. 2N26170) of the Ministry of Science and Technology.

Keywords

B-Raf
ERK
Neurite outgrowth
Nuclear translocation
PC12 cells
SB203580

ASJC Scopus subject areas

Biochemistry

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10.1023/B:BIRY.0000040206.71415.6f

Cite this

@article{d54dddf24418407aa0b7bff98106df2f,

title = "SB203580 induces prolonged B-Raf activation and promotes neuronal differentiation upon EGF treatment of PC12 cells",

abstract = "SB203580 is a p38 MAPK inhibitor that has been implicated in the activation of c-Raf. This study shows that the addition of SB203580 to PC12 cells causes the sustained activation of B-Raf but not of ERK. The addition of SB203580 prolonged the transient activation of both B-Raf and ERK by EGF alone. No significant change was detected in MAPKAPK-2 activity at low concentrations of SB203580, which induced neurite outgrowth in the EGF-stimulated PC12 cells. Therefore, these results indicate that SB203580 influences not only c-Raf as previously reported, but can also induce the activation of B-Raf, which in conjunction with EGF causes the sustained activation of ERK and differentiation in PC12 cells.",

keywords = "B-Raf, ERK, Neurite outgrowth, Nuclear translocation, PC12 cells, SB203580",

author = "Seunghee Yoon and Rony Seger and Choi, {Eui Ju} and Yoo, {Young Sook}",

note = "Funding Information: This work was supported in part by a grant from the International Cooperation Research Program (grant No. 2U02840) and Systems Biology Research Program (grant No. 2N26170) of the Ministry of Science and Technology.",

year = "2004",

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doi = "10.1023/B:BIRY.0000040206.71415.6f",

language = "English",

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T1 - SB203580 induces prolonged B-Raf activation and promotes neuronal differentiation upon EGF treatment of PC12 cells

AU - Yoon, Seunghee

AU - Seger, Rony

AU - Choi, Eui Ju

AU - Yoo, Young Sook

N1 - Funding Information: This work was supported in part by a grant from the International Cooperation Research Program (grant No. 2U02840) and Systems Biology Research Program (grant No. 2N26170) of the Ministry of Science and Technology.

PY - 2004/7

Y1 - 2004/7

N2 - SB203580 is a p38 MAPK inhibitor that has been implicated in the activation of c-Raf. This study shows that the addition of SB203580 to PC12 cells causes the sustained activation of B-Raf but not of ERK. The addition of SB203580 prolonged the transient activation of both B-Raf and ERK by EGF alone. No significant change was detected in MAPKAPK-2 activity at low concentrations of SB203580, which induced neurite outgrowth in the EGF-stimulated PC12 cells. Therefore, these results indicate that SB203580 influences not only c-Raf as previously reported, but can also induce the activation of B-Raf, which in conjunction with EGF causes the sustained activation of ERK and differentiation in PC12 cells.

AB - SB203580 is a p38 MAPK inhibitor that has been implicated in the activation of c-Raf. This study shows that the addition of SB203580 to PC12 cells causes the sustained activation of B-Raf but not of ERK. The addition of SB203580 prolonged the transient activation of both B-Raf and ERK by EGF alone. No significant change was detected in MAPKAPK-2 activity at low concentrations of SB203580, which induced neurite outgrowth in the EGF-stimulated PC12 cells. Therefore, these results indicate that SB203580 influences not only c-Raf as previously reported, but can also induce the activation of B-Raf, which in conjunction with EGF causes the sustained activation of ERK and differentiation in PC12 cells.

KW - B-Raf

KW - ERK

KW - Neurite outgrowth

KW - Nuclear translocation

KW - PC12 cells

KW - SB203580

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DO - 10.1023/B:BIRY.0000040206.71415.6f

M3 - Article

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AN - SCOPUS:4444255796

SN - 0006-2979

VL - 69

SP - 799

EP - 805

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

IS - 7

ER -

SB203580 induces prolonged B-Raf activation and promotes neuronal differentiation upon EGF treatment of PC12 cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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