Scalable Functionalization of Polyaniline-Grafted rGO Field-Effect Transistors for a Highly Sensitive Enzymatic Acetylcholine Biosensor

Dongsung Park, Dongtak Lee, Hye Jin Kim, Dae Sung Yoon, Kyo Seon Hwang

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    For decades, acetylcholine (Ach) has been considered a critical biomarker for several degenerative brain diseases, including Alzheimer’s, Parkinson’s disease, Huntington’s disease, and schizophrenia. Here, we propose a wafer-scale fabrication of polyaniline (PAni)-grafted graphene-based field-effect transistors (PGFET) and their biosensing applications for highly sensitive and reliable real-time monitoring of Ach in flow configuration. The grafted PAni provides suitable electrostatic binding sites for enzyme immobilization and enhances the pH sensitivity (2.68%/pH), compared to that of bare graphene-FET (1.81%/pH) for a pH range of 3–9 without any pH-hysteresis. We further evaluated the PGFET’s sensing performance for Ach detection with a limit of detection at the nanomolar level and significantly improved sensitivity (~103%) in the concentration range of 108 nM to 2 mM. Moreover, the PGFET exhibits excellent selectivity against various interferences, including glucose, ascorbic acid, and neurotransmitters dopamine and serotonin. Finally, we investigated the effects of an inhibitor (rivastigmine) on the AchE activity of the PGFET. From the results, we demonstrated that the PGFET has great potential as a real-time drug-screening platform by monitoring the inhibitory effects on enzymatic activity.

    Original languageEnglish
    Article number279
    JournalBiosensors
    Volume12
    Issue number5
    DOIs
    Publication statusPublished - 2022 May

    Bibliographical note

    Publisher Copyright:
    © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

    Keywords

    • acetylcholine
    • acetylcholinesterase
    • drug screening
    • field-effect transistor (FET)
    • pH-sensing
    • polyaniline
    • reduced graphene oxide

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Instrumentation
    • Engineering (miscellaneous)
    • Biotechnology
    • Biomedical Engineering
    • Clinical Biochemistry

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