Screening of oleaginous algal strains from Chlamydomonas reinhardtii mutant libraries via density gradient centrifugation

Young Joon Sung, Hong Il Choi, Jeong Seop Lee, Min Eui Hong, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Microalgae have a high potential to be utilized as feedstock for biofuels because they have high growth rates and do not compromise food production. Commercialized algae-based biofuel production relies on the development of strains with high lipid content. Based on the relatively low density of lipids compared to other cellular components, density gradient centrifugation was used to isolate high lipid content algal strains from Chlamydomonas reinhardtii mutant libraries. The correlation between cell density and lipid content was confirmed by analysis of Nile red fluorescence intensity, total lipids, and total fatty acid methyl ester content. A strain isolated by this screening method had 50% higher lipid content and 7% lower cell density than the parent wild-type strain. Consequently, we demonstrated that screening of algal strains with low cell density via continuous density gradient centrifugation allows simple, rapid, and inexpensive screening for high lipid content strains.

Original languageEnglish
Pages (from-to)3179-3188
Number of pages10
JournalBiotechnology and Bioengineering
Issue number12
Publication statusPublished - 2019 Dec 1

Bibliographical note

Funding Information:
This study was supported by the Korea CCS R&D Center (Korea CCS 2020 Project) of the National Research Foundation of Korea (NRF) (grant number: 2014M1A8A1049278); the NRF (grant number: NRF-2019R1A2C3009821/2010-0027955); and the Korea Institute of Energy Technology Evaluation and Planning (KETEP) (grant number: 20172010202050).

Publisher Copyright:
© 2019 Wiley Periodicals, Inc.


  • cell screening system
  • density gradient centrifugation
  • high lipid content strain
  • microalgae

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Screening of oleaginous algal strains from Chlamydomonas reinhardtii mutant libraries via density gradient centrifugation'. Together they form a unique fingerprint.

Cite this