Secretion of particles of hepatitis B surface antigen from insect cells using a baculovirus vector.

C. Y. Kang; D. H. Bishop; J. S. Seo; Y. Matsuura; M. Choe

doi:10.1099/0022-1317-68-10-2607

Secretion of particles of hepatitis B surface antigen from insect cells using a baculovirus vector.

C. Y. Kang, D. H. Bishop, J. S. Seo, Y. Matsuura, M. Choe

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The coding sequences of the hepatitis B virus surface antigen were inserted into a baculovirus transfer vector produced from Autographa californica nuclear polyhedrosis virus (AcNPV) so that the foreign gene was under the control of the AcNPV polyhedrin promoter. Spodoptera frugiperda cells infected with the derived recombinant baculovirus produced and secreted 22 nm particles containing the hepatitis B surface antigen. The particles had morphological and antigenic properties identical to those of 22 nm particles isolated from the plasma of chronic active hepatitis patients.

Original language	English
Pages (from-to)	2607-2613
Number of pages	7
Journal	The Journal of general virology
Volume	68 ( Pt 10)
DOIs	https://doi.org/10.1099/0022-1317-68-10-2607
Publication status	Published - 1987 Oct
Externally published	Yes

ASJC Scopus subject areas

Virology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1099/0022-1317-68-10-2607

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abstract = "The coding sequences of the hepatitis B virus surface antigen were inserted into a baculovirus transfer vector produced from Autographa californica nuclear polyhedrosis virus (AcNPV) so that the foreign gene was under the control of the AcNPV polyhedrin promoter. Spodoptera frugiperda cells infected with the derived recombinant baculovirus produced and secreted 22 nm particles containing the hepatitis B surface antigen. The particles had morphological and antigenic properties identical to those of 22 nm particles isolated from the plasma of chronic active hepatitis patients.",

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AU - Bishop, D. H.

AU - Seo, J. S.

AU - Matsuura, Y.

AU - Choe, M.

PY - 1987/10

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AB - The coding sequences of the hepatitis B virus surface antigen were inserted into a baculovirus transfer vector produced from Autographa californica nuclear polyhedrosis virus (AcNPV) so that the foreign gene was under the control of the AcNPV polyhedrin promoter. Spodoptera frugiperda cells infected with the derived recombinant baculovirus produced and secreted 22 nm particles containing the hepatitis B surface antigen. The particles had morphological and antigenic properties identical to those of 22 nm particles isolated from the plasma of chronic active hepatitis patients.

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U2 - 10.1099/0022-1317-68-10-2607

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JO - Journal of General Virology

JF - Journal of General Virology

ER -

Secretion of particles of hepatitis B surface antigen from insect cells using a baculovirus vector.

Abstract

ASJC Scopus subject areas

UN SDGs

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