Secretory profiles and wound healing effects of human amniotic fluid-derived mesenchymal stem cells

Byung Sun Yoon, Jai Hee Moon, Eun Kyoung Jun, Jonggun Kim, Isaac Maeng, Jun Sung Kim, Jung Han Lee, Cheong Soon Baik, Aeree Kim, Kyoung Shik Cho, Jang Ho Lee, Hwang Heui Lee, Kwang Youn Whang, Seungkwon You

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164 Citations (Scopus)


Recent evidence shows that amniotic fluid (AF) contains multiple cell types derived from the developing fetus, and may represent a novel source of stem cells for cell therapy. In this study, we examined the paracrine factors released by human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) and their ability to accelerate the wound-healing process by stimulating proliferation and migration of dermal fibroblasts. AF-MSCs expressed the typical MSC marker proteins CD13, CD29, and CD44 and differentiated into adipocytes, osteoblasts, and chondrocytes when exposed to the appropriate differentiation media. In addition, AF-MSC-conditioned media (AF-MSC-CM) significantly enhanced proliferation of dermal fibroblasts. Antibody-based protein array and enzyme-linked immunosorbent assay (ELISA) indicated that AF-MSC-CM contains various cytokines and chemokines that are known to be important in normal wound healing, including IL-8, IL-6, TGF-β, TNFRI, VEGF, and EGF. Application of AF-MSC-CM significantly enhanced wound healing by dermal fibroblasts via the TGF-β/SMAD2 pathway. Levels of p-SMAD2 were increased by AF-MSC-CM, and both the increase in p-SMAD2 and migration of dermal fibroblasts were blocked by inhibiting the TGF-β/SMAD2 pathway. Moreover, in a mouse excisional wound model, AF-MSC-CM accelerated wound healing. These data provide the first evidence of the potential for AF-MSC-CM in the treatment of skin wounds.

Original languageEnglish
Pages (from-to)887-902
Number of pages16
JournalStem cells and development
Issue number6
Publication statusPublished - 2010 Jun 1

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology


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