See-through technology for biological tissue: 3-dimensional visualization of macromolecules

Eunsoo Lee; Hyun Jung Kim; Woong Sun

doi:10.5213/inj.1632630.315

See-through technology for biological tissue: 3-dimensional visualization of macromolecules

Eunsoo Lee, Hyun Jung Kim, Woong Sun

Research output: Contribution to journal › Review article › peer-review

19 Citations (Scopus)

Abstract

Tissue clearing technology is currently one of the fastest growing fields in biomedical sciences. Tissue clearing techniques have become a powerful approach to understand further the structural information of intact biological tissues. Moreover, technological improvements in tissue clearing and optics allowed the visualization of neural network in the whole brain tissue with subcellular resolution. Here, we described an overview of various tissue-clearing techniques, with focus on the tissue-hydrogel mediated clearing methods, and discussed the main advantages and limitations of transparent tissue for clinical diagnosis.

Original language	English
Pages (from-to)	S15-S22
Journal	International Neurourology Journal
Volume	20
DOIs	https://doi.org/10.5213/inj.1632630.315
Publication status	Published - 2016 May 1

Bibliographical note

Funding Information:
This work was supported by the Brain Research Program through the National Research Foundation (NRF) funded by the Korean Ministry of Science, ICT and Future Planning (NRF-2015M3C7A1028790).

Publisher Copyright:
© 2016 Korean Continence Society.

Keywords

Imaging
Immunolabeling
Molecular diagnosis
Neuroscience
Optical tissue clearing
Three-dimensional

ASJC Scopus subject areas

Neurology
Clinical Neurology
Urology

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10.5213/inj.1632630.315

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title = "See-through technology for biological tissue: 3-dimensional visualization of macromolecules",

abstract = "Tissue clearing technology is currently one of the fastest growing fields in biomedical sciences. Tissue clearing techniques have become a powerful approach to understand further the structural information of intact biological tissues. Moreover, technological improvements in tissue clearing and optics allowed the visualization of neural network in the whole brain tissue with subcellular resolution. Here, we described an overview of various tissue-clearing techniques, with focus on the tissue-hydrogel mediated clearing methods, and discussed the main advantages and limitations of transparent tissue for clinical diagnosis.",

keywords = "Imaging, Immunolabeling, Molecular diagnosis, Neuroscience, Optical tissue clearing, Three-dimensional",

author = "Eunsoo Lee and Kim, {Hyun Jung} and Woong Sun",

note = "Funding Information: This work was supported by the Brain Research Program through the National Research Foundation (NRF) funded by the Korean Ministry of Science, ICT and Future Planning (NRF-2015M3C7A1028790). Publisher Copyright: {\textcopyright} 2016 Korean Continence Society.",

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doi = "10.5213/inj.1632630.315",

language = "English",

volume = "20",

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T1 - See-through technology for biological tissue

T2 - 3-dimensional visualization of macromolecules

AU - Lee, Eunsoo

AU - Kim, Hyun Jung

AU - Sun, Woong

N1 - Funding Information: This work was supported by the Brain Research Program through the National Research Foundation (NRF) funded by the Korean Ministry of Science, ICT and Future Planning (NRF-2015M3C7A1028790). Publisher Copyright: © 2016 Korean Continence Society.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Tissue clearing technology is currently one of the fastest growing fields in biomedical sciences. Tissue clearing techniques have become a powerful approach to understand further the structural information of intact biological tissues. Moreover, technological improvements in tissue clearing and optics allowed the visualization of neural network in the whole brain tissue with subcellular resolution. Here, we described an overview of various tissue-clearing techniques, with focus on the tissue-hydrogel mediated clearing methods, and discussed the main advantages and limitations of transparent tissue for clinical diagnosis.

AB - Tissue clearing technology is currently one of the fastest growing fields in biomedical sciences. Tissue clearing techniques have become a powerful approach to understand further the structural information of intact biological tissues. Moreover, technological improvements in tissue clearing and optics allowed the visualization of neural network in the whole brain tissue with subcellular resolution. Here, we described an overview of various tissue-clearing techniques, with focus on the tissue-hydrogel mediated clearing methods, and discussed the main advantages and limitations of transparent tissue for clinical diagnosis.

KW - Imaging

KW - Immunolabeling

KW - Molecular diagnosis

KW - Neuroscience

KW - Optical tissue clearing

KW - Three-dimensional

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DO - 10.5213/inj.1632630.315

M3 - Review article

AN - SCOPUS:84973483423

SN - 2093-4777

VL - 20

SP - S15-S22

JO - International Neurourology Journal

JF - International Neurourology Journal

ER -

See-through technology for biological tissue: 3-dimensional visualization of macromolecules

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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