Selection and Characterization of Probiotic Bacteria Exhibiting Antiadipogenic Potential in 3T3-L1 Preadipocytes

Chul Sang Lee, Mi Hyun Park, Sae Hun Kim

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Abnormal adipocyte growth, distinguished by an increase in cell numbers and cellular differentiation, is regarded as a major pathological characteristic of obesity. Thus, inhibition of adipogenic differentiation in adipocytes could prevent obesity. Recently, certain probiotic stains have been reported to regulate lipid metabolism in vitro and/or in vivo. In this backdrop, this study aimed to investigate basic probiotic properties and potential antiobesity characteristics of mouse 3T3-L1 preadipocytes. Six lactic acid bacteria (LAB) strains were prescreened for their cholesterol-lowering activity, antioxidant activity, and survival at low pH and in a solution containing bile salts. These six strains were investigated for antiadipogenic activity by employing 3T3-L1 mouse preadipocytes. 3T3-L1 cells were treated with selected strains during the differentiation process. Lactobacillus johnsonii 3121 and Lactobacillus rhamnosus 86 were found to be more capable of reducing triglyceride and lipid accumulation, as compared to control group, which are fully differentiated 3T3-L1 adipocytes. These strains also inhibited adipocyte differentiation by downregulating the adipogenic transcription factor in 3T3-L1 adipocytes. Taken together, these results indicate that L. johnsonni 3121 and L. rhamnosus 86 could potentially act as probiotic bacteria and prevent fat accumulation by regulating adipogenesis-related markers.

Original languageEnglish
Pages (from-to)72-86
Number of pages15
JournalProbiotics and Antimicrobial Proteins
Issue number1
Publication statusPublished - 2022 Feb

Bibliographical note

Funding Information:
This work was financially supported by grants funded by the Chong Kun Dang Bio and Korea University Grant.

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.


  • 3T3-L1 preadipocytes
  • Adipogenesis
  • Lactic acid bacteria
  • Obesity
  • Probiotics

ASJC Scopus subject areas

  • Microbiology
  • Molecular Medicine
  • Molecular Biology


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