TY - JOUR
T1 - Selective colorimetric urine glucose detection by paper sensor functionalized with polyaniline nanoparticles and cell membrane
AU - Lee, Taeha
AU - Kim, Insu
AU - Cheong, Da Yeon
AU - Roh, Seokbeom
AU - Jung, Hyo Gi
AU - Lee, Sang Won
AU - Kim, Hyun Soo
AU - Yoon, Dae Sung
AU - Hong, Yoochan
AU - Lee, Gyudo
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/5/8
Y1 - 2021/5/8
N2 - For the diabetes diagnosis, noninvasive methods are preferred to invasive methods; urine glucose measurement is an example of a noninvasive method. However, conventional noninvasive methods for urine glucose measurement are not intuitive. Furthermore, such methods exhibit low selectivity because they can detect interfering molecules in addition to glucose. Herein, we fabricate a noninvasive, intuitive, and highly selective paper sensor consisting of polyaniline nanoparticles (PAni-NPs) and red blood cell membranes (RBCMs). The PAni-NPs (adsorbed on the paper) are highly sensitive to hydrogen ions and change color from emeraldine blue to emeraldine green within a few seconds. The RBCM (coated on the PAni-NP-adsorbed paper) having the glucose transporter-1 protein plays the role of a smart filter that transports glucose but rejects other interfering molecules. In particular, the selectivity of the RBCM-coated PAni-NP-based paper sensor was approximately improved ∼85%, compared to the uncoated paper sensors. The paper sensor could detect urine glucose over the range of 0–10 mg/mL (0–56 mM), with a limit of detection of 0.54 mM. The proposed paper sensor will facilitate the development of a highly selective and colorimetric urine glucose monitoring system.
AB - For the diabetes diagnosis, noninvasive methods are preferred to invasive methods; urine glucose measurement is an example of a noninvasive method. However, conventional noninvasive methods for urine glucose measurement are not intuitive. Furthermore, such methods exhibit low selectivity because they can detect interfering molecules in addition to glucose. Herein, we fabricate a noninvasive, intuitive, and highly selective paper sensor consisting of polyaniline nanoparticles (PAni-NPs) and red blood cell membranes (RBCMs). The PAni-NPs (adsorbed on the paper) are highly sensitive to hydrogen ions and change color from emeraldine blue to emeraldine green within a few seconds. The RBCM (coated on the PAni-NP-adsorbed paper) having the glucose transporter-1 protein plays the role of a smart filter that transports glucose but rejects other interfering molecules. In particular, the selectivity of the RBCM-coated PAni-NP-based paper sensor was approximately improved ∼85%, compared to the uncoated paper sensors. The paper sensor could detect urine glucose over the range of 0–10 mg/mL (0–56 mM), with a limit of detection of 0.54 mM. The proposed paper sensor will facilitate the development of a highly selective and colorimetric urine glucose monitoring system.
KW - Colorimetric paper sensor
KW - High selectivity
KW - Noninvasive
KW - Polyaniline nanoparticles
KW - Red blood cell membrane
KW - Urine glucose
UR - http://www.scopus.com/inward/record.url?scp=85103592444&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2021.338387
DO - 10.1016/j.aca.2021.338387
M3 - Article
C2 - 33863418
AN - SCOPUS:85103592444
SN - 0003-2670
VL - 1158
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
M1 - 338387
ER -