Abstract
The metabolic engineering of epothilones, as secondary metabolites, was investigated using Sorangium cellulosum to achieve the selective production of epothilone B, a potent anticancer agent. Thus, the propionyl-CoA synthetase gene (prpE) from Ralstonia solanacearum was heterologously expressed in S. cellulosum to increase the production of epothilone B. Propionyl-CoA synthetase converts propionate into propionyl-CoA, a potent precursor of epothilone B. The recombinant S. cellulosum containing the prpE gene exhibited a significant increase in the resolution of epothilones B/A, with an epothilone B to A ratio of 127 to 1, which was 100 times higher than that of the wild-type cells, demonstratin its potential use for the selective production of epothilone B.
Original language | English |
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Pages (from-to) | 135-137 |
Number of pages | 3 |
Journal | Journal of microbiology and biotechnology |
Volume | 18 |
Issue number | 1 |
Publication status | Published - 2008 Jan 28 |
Externally published | Yes |
Keywords
- Epothilone
- Heterologous expression
- Metabolic engineering
- Propionyl-CoA synthetase
- Sorangium cellulosum
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology