Self-assemble nanostructured ensembles for detection of guanosine triphosphate based on receptor structure modulated sensitivity and selectivity

Qian Yong Cao, Ling Xi Huang, Seyoung Koo, Yue Bo He, Paramesh Jangili, Jong Seung Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


As one vital member of the biologically important nucleotides, guanosine-5′-triphosphate (GTP) monitoring in biological systems has become a key research field because its abnormal concentration promotes certain pathological states. Herein, we report two “cleft-form” amphiphilic pyridinium receptors based on isophthalamide (PyPh-C16) and 2,6-dicarboxamidopyridine (PyPy-C16) spacers for structure modulated sensitive and selective response to GTP via the indicator displacement assay (IDA) method. Both receptors bind with a fluorescent indicator uranine dye (UD) to form self-assembled non-fluorescent nano-aggregate ensembles, i.e., PyPh-C16/UD and PyPy-C16/UD, which can be used for the nM detection of GTP in aqueous solution and also imaging GTP in complicated living cells environment. The non-fluorescent UD becomes fluorescent with releasing from the nano-ensembles displaced by GTP. With the aid of this phenomenon, the nano-ensembles could successfully discriminate GTP from other structurally related anions, including ATP, PPi, and most nucleotide polyphosphates (NPPs). In addition, the structure-related sensing properties of receptors toward GTP were detailly discussed. Thus, we believe that the present work will allow for many important developments in the future based on IDAs with advanced nano-ensembles and different indicators to recognize various types of essential nucleoside polyphosphates.

Original languageEnglish
Article number132091
JournalSensors and Actuators, B: Chemical
Publication statusPublished - 2022 Oct 1

Bibliographical note

Funding Information:
This work was supported by the National Nature Science Foundation of China (nos. 22061028 and 21762028 ) and a CRI project (Grant No. 2018R1A3B1052702 , JSK) of the NRF in Korea and Basic Science Research Programs ( 2022R1C1C2007637 , SK) of the NRF in Korea. This work was also supported by the Korea University Grant (PJ).

Publisher Copyright:
© 2022 Elsevier B.V.


  • Amphiphilic receptors
  • Detection of GTP
  • Fluorescence sensor
  • Indicator displacement assay
  • Self-assembly

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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