Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production

Sang Il Han; Min Seo Jeon; Yun Hwan Park; Sok Kim; Yoon E. Choi

doi:10.1016/j.biortech.2021.125816

Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production

Sang Il Han, Min Seo Jeon, Yun Hwan Park, Sok Kim, Yoon E. Choi

Division of Environmental Science and Ecological Engineering

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production. The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively. Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control. Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control. The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control. This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.

Original language	English
Article number	125816
Journal	Bioresource technology
Volume	341
DOIs	https://doi.org/10.1016/j.biortech.2021.125816
Publication status	Published - 2021 Dec

Keywords

Calcium alginate bead
Immobilized culture
Porphyridium cruentum
Semi-continuous culture
Sulfated polysaccharides

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2021.125816

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@article{168ef563c03f4f3d99fc9c2019746d79,

title = "Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production",

abstract = "In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production. The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively. Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control. Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control. The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control. This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.",

keywords = "Calcium alginate bead, Immobilized culture, Porphyridium cruentum, Semi-continuous culture, Sulfated polysaccharides",

author = "Han, {Sang Il} and Jeon, {Min Seo} and Park, {Yun Hwan} and Sok Kim and Choi, {Yoon E.}",

note = "Funding Information: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2019R1A2C2087449) and the Ministry of Education (2021R1A6A3A01087397). This research was also supported by Korea University Future Research Grant and the Commercializations Promotion Agency for R&D Outcomes (COMPA) grant funded by the Korea government (MSIT) (No. 2021B100). Publisher Copyright: {\textcopyright} 2021",

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doi = "10.1016/j.biortech.2021.125816",

language = "English",

volume = "341",

journal = "Bioresource Technology",

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T1 - Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production

AU - Han, Sang Il

AU - Jeon, Min Seo

AU - Park, Yun Hwan

AU - Kim, Sok

AU - Choi, Yoon E.

N1 - Funding Information: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (2019R1A2C2087449) and the Ministry of Education (2021R1A6A3A01087397). This research was also supported by Korea University Future Research Grant and the Commercializations Promotion Agency for R&D Outcomes (COMPA) grant funded by the Korea government (MSIT) (No. 2021B100). Publisher Copyright: © 2021

PY - 2021/12

Y1 - 2021/12

N2 - In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production. The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively. Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control. Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control. The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control. This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.

AB - In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production. The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively. Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control. Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control. The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control. This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.

KW - Calcium alginate bead

KW - Immobilized culture

KW - Porphyridium cruentum

KW - Semi-continuous culture

KW - Sulfated polysaccharides

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SN - 0960-8524

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JO - Bioresource Technology

JF - Bioresource Technology

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Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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