Abstract
A label-free and high-sensitive sensing method of cancer DNA mutations using QCM(quartz crystal microbalance) is developed. The detection is based on the evaluation of the resonance frequecny. When probe DNA (pDNA) is introduced on quartz of sensor of QCM, the resonance frequency is shifted. When pDNA captures target mutant DNA through DNA hybridization, an additional resonance frequency shift occurs due to mass change in association with target DNA. The platform is able to sense EGFR, lung cancer mutant DNA with a limit of detection (LOD) of 1.0 nM.
| Original language | English |
|---|---|
| Publication status | Published - 2017 |
| Event | 24th International Congress on Sound and Vibration, ICSV 2017 - London, United Kingdom Duration: 2017 Jul 23 → 2017 Jul 27 |
Other
| Other | 24th International Congress on Sound and Vibration, ICSV 2017 |
|---|---|
| Country/Territory | United Kingdom |
| City | London |
| Period | 17/7/23 → 17/7/27 |
Bibliographical note
Funding Information:This work was supported by the National Research Foundation of Korea (NRF) under Grant Number NRF-2007-0056094 and NRF-2014R1A2A1A11052389 which are funded by the Ministry of Science, ICT & Future Planning.
Keywords
- Epidermal growth factor receptor (EGFR)
- In situ detection
- Mutation
- Nano-porous
- Quartz crystal microbalance (QCM)
ASJC Scopus subject areas
- Acoustics and Ultrasonics
