Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays

Su Jin Lee; Jee Woong Park; In Ae Kim; Byung Soo Youn; Man Bock Gu

doi:10.1016/j.snb.2012.04.016

Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays

Su Jin Lee, Jee Woong Park, In Ae Kim, Byung Soo Youn, Man Bock Gu

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

We developed an enzyme-linked antibody-ssDNA aptamer sandwich (ELAAS) method to detect three adipokines for diagnosing type 2 diabetes. For this application, three different aptamers for retinol-binding protein-4 (RBP4), visceral adipose tissue-derived serpin (Vaspin), and nicotinamide phosphoribosyltransferase (Nampt/visfatin) were used as capturing probes, and enzyme-linked polyclonal antibodies were used for signal generation. The sensitivities of this method for all three proteins were enhanced by at least 20-fold to up to 68-fold higher than that of the surface plasmon resonance (SPR) system, which only uses aptamers as capturing probes. In addition, this assay method exhibited equal sensitivity in the detection of each adipokine spiked in diluted human serum. In particular, the detection limit for RBP4 was similar to that obtained by western blotting, as reported previously. The simplicity, reproducibility, and high sensitivity of the ELAAS method could allow it to be extensively utilized in field.

Original language	English
Pages (from-to)	243-248
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	168
DOIs	https://doi.org/10.1016/j.snb.2012.04.016
Publication status	Published - 2012 Jun 20

Bibliographical note

Funding Information:
This work was supported in part by both the Advanced Production Technology Development Program for Agriculture and Forestry in the Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea and the Technical Transfer funded by the Small & Medium Business Administration (SMBA) (Project #S1071611). The authors thank Ms. Hye-Jin Hong at Korea University for her assistance in preparing reagents and technical help in performing the assays.

Keywords

Adipokines
Antibodies
Aptamers
ELAAS biosensor
Type 2 diabetes

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2012.04.016

Cite this

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title = "Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays",

abstract = "We developed an enzyme-linked antibody-ssDNA aptamer sandwich (ELAAS) method to detect three adipokines for diagnosing type 2 diabetes. For this application, three different aptamers for retinol-binding protein-4 (RBP4), visceral adipose tissue-derived serpin (Vaspin), and nicotinamide phosphoribosyltransferase (Nampt/visfatin) were used as capturing probes, and enzyme-linked polyclonal antibodies were used for signal generation. The sensitivities of this method for all three proteins were enhanced by at least 20-fold to up to 68-fold higher than that of the surface plasmon resonance (SPR) system, which only uses aptamers as capturing probes. In addition, this assay method exhibited equal sensitivity in the detection of each adipokine spiked in diluted human serum. In particular, the detection limit for RBP4 was similar to that obtained by western blotting, as reported previously. The simplicity, reproducibility, and high sensitivity of the ELAAS method could allow it to be extensively utilized in field.",

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note = "Funding Information: This work was supported in part by both the Advanced Production Technology Development Program for Agriculture and Forestry in the Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea and the Technical Transfer funded by the Small & Medium Business Administration (SMBA) (Project #S1071611). The authors thank Ms. Hye-Jin Hong at Korea University for her assistance in preparing reagents and technical help in performing the assays. ",

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AU - Gu, Man Bock

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N2 - We developed an enzyme-linked antibody-ssDNA aptamer sandwich (ELAAS) method to detect three adipokines for diagnosing type 2 diabetes. For this application, three different aptamers for retinol-binding protein-4 (RBP4), visceral adipose tissue-derived serpin (Vaspin), and nicotinamide phosphoribosyltransferase (Nampt/visfatin) were used as capturing probes, and enzyme-linked polyclonal antibodies were used for signal generation. The sensitivities of this method for all three proteins were enhanced by at least 20-fold to up to 68-fold higher than that of the surface plasmon resonance (SPR) system, which only uses aptamers as capturing probes. In addition, this assay method exhibited equal sensitivity in the detection of each adipokine spiked in diluted human serum. In particular, the detection limit for RBP4 was similar to that obtained by western blotting, as reported previously. The simplicity, reproducibility, and high sensitivity of the ELAAS method could allow it to be extensively utilized in field.

AB - We developed an enzyme-linked antibody-ssDNA aptamer sandwich (ELAAS) method to detect three adipokines for diagnosing type 2 diabetes. For this application, three different aptamers for retinol-binding protein-4 (RBP4), visceral adipose tissue-derived serpin (Vaspin), and nicotinamide phosphoribosyltransferase (Nampt/visfatin) were used as capturing probes, and enzyme-linked polyclonal antibodies were used for signal generation. The sensitivities of this method for all three proteins were enhanced by at least 20-fold to up to 68-fold higher than that of the surface plasmon resonance (SPR) system, which only uses aptamers as capturing probes. In addition, this assay method exhibited equal sensitivity in the detection of each adipokine spiked in diluted human serum. In particular, the detection limit for RBP4 was similar to that obtained by western blotting, as reported previously. The simplicity, reproducibility, and high sensitivity of the ELAAS method could allow it to be extensively utilized in field.

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Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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