Sensitive, selective and resuable electrochemical sensor for mercury(II) based on molecular beacon approach

Donghoon Han, Yang Rae Kim, Jeong Wook Oh, Jong Seung Kim, Hasuck Kim

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We have developed a sensitive and selective electrochemical sensor for the detection of mercury(II) ions in aqueous media. This electrochemical sensor is based on the molecular beacon which involves an electroactive, ferrocene-labeled DNA hairpin structure and provides strategically selective binding of thyminethymine mismatch for Hg2+ ions. The modified electrode showed a voltammetric signal due to an one-step redox reaction of the surface-confined ferrocenyl moiety. The "signal-on" upon mercury binding could be attributed to a change in conformation from open structure to restricted hairpin structure. The molecular beacon mercury(II) ion sensor was amenable to regeneration by unfolding the ferrocene-labeled DNA in 10 μM cysteine.

    Original languageEnglish
    Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherChemical and Biological Microsystems Society
    Pages964-966
    Number of pages3
    ISBN (Print)9780979806421
    Publication statusPublished - 2009
    Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
    Duration: 2009 Nov 12009 Nov 5

    Publication series

    NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

    Other

    Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
    Country/TerritoryKorea, Republic of
    CityJeju
    Period09/11/109/11/5

    Keywords

    • Electrochemical detection

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

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