Separation of the antioxidant compound quercitrin from Lindera obtusiloba blume and its antimelanogenic effect on B16F10 melanoma cells

Chung Oui Hong; Hyun Ah Lee; Chae Hong Rhee; Se Young Choung; Kwang Won Lee

doi:10.1271/bbb.120562

Separation of the antioxidant compound quercitrin from Lindera obtusiloba blume and its antimelanogenic effect on B16F10 melanoma cells

Chung Oui Hong
, Hyun Ah Lee
, Chae Hong Rhee
, Se Young Choung
, Kwang Won Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Considering the growing evidence of the presence of antioxidant compounds in plant extracts, the objectives of this study were to identify antioxidant compounds in Lindera obtusiloba Blume (Lauraceae) and to evaluate their antimelanogenic activities on B16F10 melanoma cells. Organic solvent fractions were separated from L. obtusiloba extracts (LOE). The ethyl acetate fraction (LOE-E) was significantly active against oxidative damage induced by tert-butyl hydroperoxide in primary rat hepatocytes. Two single purified compounds, quercitrin (quercetin-3-O-α-L-rhamnopyranoside) and afzelin (kaempferol-3-O-α-L-rhamnoside), were identified by HPLC and NMR. These compounds were evaluated for antioxidant activities by 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay, and for their antimelanogenic activities by tyrosinase inhibitory assay melanin formation inhibition assay and Western bolt analysis for the signaling pathway. The significant effects of quercitrin on antioxidant and antimelanogenic activities, and signal modulation of ERK and MITF in B16F10 melanoma cells were observed. This is the first report to identify quercitrin in L. obtusiloba and its whitening effect.

Original language	English
Pages (from-to)	58-64
Number of pages	7
Journal	Bioscience, Biotechnology and Biochemistry
Volume	77
Issue number	1
DOIs	https://doi.org/10.1271/bbb.120562
Publication status	Published - 2013

Bibliographical note

Funding Information:
This research was supported by grant nos. 109140-03-SB010 and 111137-03-1-HD120 from the High Value-Added Food Technology Development Program of the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (iPET), and the Ministry for Food, Agriculture, Forestry, and Fisheries of the Republic of Korea. We thank the Korea University-CJ Food Safety Hall and the Institute of Food and Biomedicine Safety for the use of their equipment and facilities.

Keywords

Antimelanogenic activity
Antioxidant activity
Kaempferol- 3-O-α-L-rhamnoside (afzelin)
Lindera obtusilob
Quercetin-3-O-α- L-rhamnopyranoside (quercitrin)

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.120562

Cite this

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title = "Separation of the antioxidant compound quercitrin from Lindera obtusiloba blume and its antimelanogenic effect on B16F10 melanoma cells",

abstract = "Considering the growing evidence of the presence of antioxidant compounds in plant extracts, the objectives of this study were to identify antioxidant compounds in Lindera obtusiloba Blume (Lauraceae) and to evaluate their antimelanogenic activities on B16F10 melanoma cells. Organic solvent fractions were separated from L. obtusiloba extracts (LOE). The ethyl acetate fraction (LOE-E) was significantly active against oxidative damage induced by tert-butyl hydroperoxide in primary rat hepatocytes. Two single purified compounds, quercitrin (quercetin-3-O-α-L-rhamnopyranoside) and afzelin (kaempferol-3-O-α-L-rhamnoside), were identified by HPLC and NMR. These compounds were evaluated for antioxidant activities by 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay, and for their antimelanogenic activities by tyrosinase inhibitory assay melanin formation inhibition assay and Western bolt analysis for the signaling pathway. The significant effects of quercitrin on antioxidant and antimelanogenic activities, and signal modulation of ERK and MITF in B16F10 melanoma cells were observed. This is the first report to identify quercitrin in L. obtusiloba and its whitening effect.",

keywords = "Antimelanogenic activity, Antioxidant activity, Kaempferol- 3-O-α-L-rhamnoside (afzelin), Lindera obtusilob, Quercetin-3-O-α- L-rhamnopyranoside (quercitrin)",

author = "Hong, \{Chung Oui\} and Lee, \{Hyun Ah\} and Rhee, \{Chae Hong\} and Choung, \{Se Young\} and Lee, \{Kwang Won\}",

note = "Funding Information: This research was supported by grant nos. 109140-03-SB010 and 111137-03-1-HD120 from the High Value-Added Food Technology Development Program of the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (iPET), and the Ministry for Food, Agriculture, Forestry, and Fisheries of the Republic of Korea. We thank the Korea University-CJ Food Safety Hall and the Institute of Food and Biomedicine Safety for the use of their equipment and facilities.",

year = "2013",

doi = "10.1271/bbb.120562",

language = "English",

volume = "77",

pages = "58--64",

journal = "Bioscience, Biotechnology and Biochemistry",

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publisher = "Oxford University Press",

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AU - Hong, Chung Oui

AU - Lee, Hyun Ah

AU - Rhee, Chae Hong

AU - Choung, Se Young

AU - Lee, Kwang Won

N1 - Funding Information: This research was supported by grant nos. 109140-03-SB010 and 111137-03-1-HD120 from the High Value-Added Food Technology Development Program of the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (iPET), and the Ministry for Food, Agriculture, Forestry, and Fisheries of the Republic of Korea. We thank the Korea University-CJ Food Safety Hall and the Institute of Food and Biomedicine Safety for the use of their equipment and facilities.

PY - 2013

Y1 - 2013

N2 - Considering the growing evidence of the presence of antioxidant compounds in plant extracts, the objectives of this study were to identify antioxidant compounds in Lindera obtusiloba Blume (Lauraceae) and to evaluate their antimelanogenic activities on B16F10 melanoma cells. Organic solvent fractions were separated from L. obtusiloba extracts (LOE). The ethyl acetate fraction (LOE-E) was significantly active against oxidative damage induced by tert-butyl hydroperoxide in primary rat hepatocytes. Two single purified compounds, quercitrin (quercetin-3-O-α-L-rhamnopyranoside) and afzelin (kaempferol-3-O-α-L-rhamnoside), were identified by HPLC and NMR. These compounds were evaluated for antioxidant activities by 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay, and for their antimelanogenic activities by tyrosinase inhibitory assay melanin formation inhibition assay and Western bolt analysis for the signaling pathway. The significant effects of quercitrin on antioxidant and antimelanogenic activities, and signal modulation of ERK and MITF in B16F10 melanoma cells were observed. This is the first report to identify quercitrin in L. obtusiloba and its whitening effect.

AB - Considering the growing evidence of the presence of antioxidant compounds in plant extracts, the objectives of this study were to identify antioxidant compounds in Lindera obtusiloba Blume (Lauraceae) and to evaluate their antimelanogenic activities on B16F10 melanoma cells. Organic solvent fractions were separated from L. obtusiloba extracts (LOE). The ethyl acetate fraction (LOE-E) was significantly active against oxidative damage induced by tert-butyl hydroperoxide in primary rat hepatocytes. Two single purified compounds, quercitrin (quercetin-3-O-α-L-rhamnopyranoside) and afzelin (kaempferol-3-O-α-L-rhamnoside), were identified by HPLC and NMR. These compounds were evaluated for antioxidant activities by 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay, and for their antimelanogenic activities by tyrosinase inhibitory assay melanin formation inhibition assay and Western bolt analysis for the signaling pathway. The significant effects of quercitrin on antioxidant and antimelanogenic activities, and signal modulation of ERK and MITF in B16F10 melanoma cells were observed. This is the first report to identify quercitrin in L. obtusiloba and its whitening effect.

KW - Antimelanogenic activity

KW - Antioxidant activity

KW - Kaempferol- 3-O-α-L-rhamnoside (afzelin)

KW - Lindera obtusilob

KW - Quercetin-3-O-α- L-rhamnopyranoside (quercitrin)

UR - https://www.scopus.com/pages/publications/84873547092

U2 - 10.1271/bbb.120562

DO - 10.1271/bbb.120562

M3 - Article

C2 - 23291772

AN - SCOPUS:84873547092

SN - 0916-8451

VL - 77

SP - 58

EP - 64

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 1

ER -

Separation of the antioxidant compound quercitrin from Lindera obtusiloba blume and its antimelanogenic effect on B16F10 melanoma cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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