Serinicoccus sediminis sp. nov., isolated from tidal flat sediment

A novel Gram-strain-positive, non-spore-forming bacterial strain, designated GP-T3-3T, was isolated from sediment sampled at a tidal flat in Gopado, Republic of Korea. Cells were aerobic, catalase-negative, oxidase-positive, non-motile cocci that occurred singly, in pairs or in clusters. Strain GP-T3-3T grew at 4-45 °C (optimum, 28-37 °C), at pH 4.0-12.0 (pH 8.0-9.0) and in the presence of 0-15% (w/v) NaCl (3-5%). Colonies of strain GP-T3-3T were deep-yellow, circular, smooth and pulvinate. The results of the phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GP-T3-3T was closely related to Serinicoccus profundi MCCC 1A05965^T (99.1%), Serinicoccus chungangensis CAU 9536^T (99.0%) and Serinicoccus marinus JC1078^T (98.0%). The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant respiratory quinone was MK-8(H₄) and the major fatty acids were anteiso-C_{17: 0}, iso-C_{16: 0}, iso-C_{15: 0} and anteiso-C_{15: 0}. The polar lipid profile consisted of diphosphadidylglycerol, phosphadidylglycerol, phosphatidylcholine, phosphatidylinositol and two unidentified phospholipids. The DNA G+C content was 72.9 mol%. DNA-DNA relatedness values between strain GP-T3-3T and type strains of the genus Serinicoccus ranged from 28.9 to 50.5%. On the basis of the phenotypic differences and DNA-DNA relatedness data, the isolate represents a new species of the genus Serinicoccus, for which the name Serinicoccus sediminis sp. nov. is proposed. The type strain is GP-T3-3T (=KCTC 49173^T=JCM 32825^T=KCCM 43309^T=KACC 19850^T).