SERS-based particle tracking and molecular imaging in live cells: Toward the monitoring of intracellular dynamics

Jongwoo Kim, Sang Hwan Nam, Dong Kwon Lim, Yung Doug Suh

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Although diverse endogenous biomolecules involved in life processes are of major interest in cell biology, there is still a lack of suitable methods for studying biomolecules within live cells without labelling. Herein, we describe a near-infrared (NIR) surface-enhanced Raman scattering (SERS)-based particle tracking technique gathering chemical information inside live cells for monitoring their intracellular dynamics. The wide-field SERS imaging spectroscopy system facilitates high temporal resolution (200 ms) under high spatial resolution (512 × 512 pixels) for one live cell. With high spatiotemporal resolution and signal-to-background ratio, we show that the Raman signal from intracellular cargoes in live cells is sporadically observed and localized to a vesicular level.

Original languageEnglish
Pages (from-to)21724-21727
Number of pages4
Issue number45
Publication statusPublished - 2019 Dec 7

Bibliographical note

Funding Information:
Y. D. S. was supported by the KRICT (KK1933-10), the Global Research Laboratory (GRL) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (no. 2016911815), and the Industrial Strategic Technology Development Program (no. 10077582) funded by the Ministry of Trade, Industry, and Energy (MOTIE), Korea. This work was also supported by the National Research Council of Science & Technology (NST) grant from the Government of Korea (MSIT) (CRC-16-01-KRICT). D.-K. L. acknowledges that this work was supported by the KU-KIST School Research Fund and NRF 2018R1A2A3075499.

Publisher Copyright:
© 2019 The Royal Society of Chemistry.

ASJC Scopus subject areas

  • General Materials Science


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