SERS-based particle tracking and molecular imaging in live cells: Toward the monitoring of intracellular dynamics

Jongwoo Kim; Sang Hwan Nam; Dong Kwon Lim; Yung Doug Suh

doi:10.1039/c9nr05159g

SERS-based particle tracking and molecular imaging in live cells: Toward the monitoring of intracellular dynamics

Jongwoo Kim, Sang Hwan Nam, Dong Kwon Lim, Yung Doug Suh

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Although diverse endogenous biomolecules involved in life processes are of major interest in cell biology, there is still a lack of suitable methods for studying biomolecules within live cells without labelling. Herein, we describe a near-infrared (NIR) surface-enhanced Raman scattering (SERS)-based particle tracking technique gathering chemical information inside live cells for monitoring their intracellular dynamics. The wide-field SERS imaging spectroscopy system facilitates high temporal resolution (200 ms) under high spatial resolution (512 × 512 pixels) for one live cell. With high spatiotemporal resolution and signal-to-background ratio, we show that the Raman signal from intracellular cargoes in live cells is sporadically observed and localized to a vesicular level.

Original language	English
Pages (from-to)	21724-21727
Number of pages	4
Journal	Nanoscale
Volume	11
Issue number	45
DOIs	https://doi.org/10.1039/c9nr05159g
Publication status	Published - 2019 Dec 7

ASJC Scopus subject areas

Materials Science(all)

Access to Document

10.1039/c9nr05159g

Cite this

@article{c5179cf785224684b2bed5c541ad3a9a,

title = "SERS-based particle tracking and molecular imaging in live cells: Toward the monitoring of intracellular dynamics",

abstract = "Although diverse endogenous biomolecules involved in life processes are of major interest in cell biology, there is still a lack of suitable methods for studying biomolecules within live cells without labelling. Herein, we describe a near-infrared (NIR) surface-enhanced Raman scattering (SERS)-based particle tracking technique gathering chemical information inside live cells for monitoring their intracellular dynamics. The wide-field SERS imaging spectroscopy system facilitates high temporal resolution (200 ms) under high spatial resolution (512 × 512 pixels) for one live cell. With high spatiotemporal resolution and signal-to-background ratio, we show that the Raman signal from intracellular cargoes in live cells is sporadically observed and localized to a vesicular level.",

author = "Jongwoo Kim and Nam, {Sang Hwan} and Lim, {Dong Kwon} and Suh, {Yung Doug}",

note = "Funding Information: Y. D. S. was supported by the KRICT (KK1933-10), the Global Research Laboratory (GRL) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (no. 2016911815), and the Industrial Strategic Technology Development Program (no. 10077582) funded by the Ministry of Trade, Industry, and Energy (MOTIE), Korea. This work was also supported by the National Research Council of Science & Technology (NST) grant from the Government of Korea (MSIT) (CRC-16-01-KRICT). D.-K. L. acknowledges that this work was supported by the KU-KIST School Research Fund and NRF 2018R1A2A3075499. Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

year = "2019",

month = dec,

day = "7",

doi = "10.1039/c9nr05159g",

language = "English",

volume = "11",

pages = "21724--21727",

journal = "Nanoscale",

issn = "2040-3364",

publisher = "Royal Society of Chemistry",

number = "45",

}

TY - JOUR

T1 - SERS-based particle tracking and molecular imaging in live cells

T2 - Toward the monitoring of intracellular dynamics

AU - Kim, Jongwoo

AU - Nam, Sang Hwan

AU - Lim, Dong Kwon

AU - Suh, Yung Doug

N1 - Funding Information: Y. D. S. was supported by the KRICT (KK1933-10), the Global Research Laboratory (GRL) Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (no. 2016911815), and the Industrial Strategic Technology Development Program (no. 10077582) funded by the Ministry of Trade, Industry, and Energy (MOTIE), Korea. This work was also supported by the National Research Council of Science & Technology (NST) grant from the Government of Korea (MSIT) (CRC-16-01-KRICT). D.-K. L. acknowledges that this work was supported by the KU-KIST School Research Fund and NRF 2018R1A2A3075499. Publisher Copyright: © 2019 The Royal Society of Chemistry.

PY - 2019/12/7

Y1 - 2019/12/7

N2 - Although diverse endogenous biomolecules involved in life processes are of major interest in cell biology, there is still a lack of suitable methods for studying biomolecules within live cells without labelling. Herein, we describe a near-infrared (NIR) surface-enhanced Raman scattering (SERS)-based particle tracking technique gathering chemical information inside live cells for monitoring their intracellular dynamics. The wide-field SERS imaging spectroscopy system facilitates high temporal resolution (200 ms) under high spatial resolution (512 × 512 pixels) for one live cell. With high spatiotemporal resolution and signal-to-background ratio, we show that the Raman signal from intracellular cargoes in live cells is sporadically observed and localized to a vesicular level.

AB - Although diverse endogenous biomolecules involved in life processes are of major interest in cell biology, there is still a lack of suitable methods for studying biomolecules within live cells without labelling. Herein, we describe a near-infrared (NIR) surface-enhanced Raman scattering (SERS)-based particle tracking technique gathering chemical information inside live cells for monitoring their intracellular dynamics. The wide-field SERS imaging spectroscopy system facilitates high temporal resolution (200 ms) under high spatial resolution (512 × 512 pixels) for one live cell. With high spatiotemporal resolution and signal-to-background ratio, we show that the Raman signal from intracellular cargoes in live cells is sporadically observed and localized to a vesicular level.

UR - http://www.scopus.com/inward/record.url?scp=85075637179&partnerID=8YFLogxK

U2 - 10.1039/c9nr05159g

DO - 10.1039/c9nr05159g

M3 - Article

C2 - 31495836

AN - SCOPUS:85075637179

SN - 2040-3364

VL - 11

SP - 21724

EP - 21727

JO - Nanoscale

JF - Nanoscale

IS - 45

ER -

SERS-based particle tracking and molecular imaging in live cells: Toward the monitoring of intracellular dynamics

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this